Disease related

anti-Plakophilin 2 mouse monoclonal, Pkp2-518, supernatant

Cat. No: 651167

Available, delivery time 1-7 days

$327.00*

Key Features
  • Mouse monoclonal
  • Suitable for IEM, IHC, IP and WB
  • Reacts with bovine, human and rat
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IEM, IHC, IP, WB
Purification Hybridoma cell culture supernatant
Reactivity Human, Mouse, Rat
No reactivity Amphibia, Fish
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Pkp2-518
Immunogen Synthetic carboxyterminal peptide (aa 527 - 872) of human plakophilin 2
Formulation Contains 0.09% sodium azide
UniprotID A0A3Q1M2G3 (Bovine), Q99959 (Human), Q9CQ73 (Mouse), Q562C0 (Rat)
Synonym Plakophilin-2, PKP2
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Immuno Electron Microscopy (IEM) Assay dependent
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) Assay dependent
Background

The mab recognizes plakophilin 2 in both locations: in the desmosomal plaque and in the karyoplasm. Plakophilin 2 is a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, in Merkel cells, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the nuclei (karyoplasm).
Polypeptide reacting: Plakophilin 2, Mr ~100 kDa (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38)
Positive staining was obtained with several carcinomas derived from simple-type epithelia (e.g. hepatomas, colon carcinomas) and some adenocarcinomas derived from stratified epithelia (oesophagus carcinoma).
Recommendation for immunohistochemistry on frozen sections: For better accessibility of the desmosome-associated antigen frozen sections might be pre-incubated for 5 min in PBS plus 0.2% Triton X-100 prior to antibody application. Longer incubation could result in a loss of Pkp2-nuclear staining. To prevent wash-out of soluble forms of the antigen, fixation with 2% formaldehyde is recommended.

References/Publications (3)
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