Epitope mapped
Highly published
Diagnostic marker

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified

Liquid

Lyophilized

Liquid

Sample

Cat. No: 690029

Available, delivery time 1-7 days

$425.00*

Key Features
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human, rabbit, rat
  • Isotype: IgG2b
Product description
Quantity 1 ml (50 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b
Conjugate Unconjugated
Application ELISA, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Rabbit, Rat
No reactivity Chicken, Mouse, Woodchuck, Xenopus
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks19.2 (Z105.6)(also published as BM 19.21, MAK 19.21)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Concentration 50 µg/ml
Formulation Contains 0.09% sodium azide, 0.5 % BSA in PBS buffer, pH 7.4
UniprotID P08728 (Bovine), P08727 (Human), Q63279 (Rat)
Synonym Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10-1:50
Immunohistochemistry (IHC) - paraffin 1:10-1:50 (microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) 1:50-1:500
Background

Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma! Polypeptide Reacting: Mr 40,000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the alpha-helical fragment.

Tumors specifically detected: all tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma.

Reactivity on cultured cell lines: MCF-7.

References/Publications (19)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

anti-Keratin K19 mouse monoclonal, Ks19.10, supernatant
Cat. No: 65135
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human and rat
  • Isotype: IgG1

$191.00*
Keratin K19, human recombinant, 250 µg
Cat. No: 62014
Quantity: 250 µg
  • Recombinant human keratin K19 (formerly also designated cytokeratin 19)
  • Protein standard

Variants from $213.00*
$371.00*
anti-Keratin K19 mouse monoclonal, RCK108, supernatant
Cat. No: 11417
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
  • Reacts with human and  mammals
  • Isotype: IgG1

$285.00*
anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), lyophilized, purified
Cat. No: 61010
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG2a

Variants from $170.00*
$304.00*
anti-Keratin K19 guinea pig polyclonal, serum
Cat. No: GP-CK19
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine and human

$367.00*