anti-Plakophilin 3 guinea pig polyclonal, serum

Cat. No: GP-PP3

Available, delivery time 1-7 days

$325.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Human recombinant plakophilin 3 peptide (aa 277-474) purified from E. coli
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID Q9Y446 (Human)
Synonym Plakophilin-3, PKP3
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 (also after paraformaldehyde fixation)
Western Blot (WB) 1:1,000
Background

PP3-1 antiserum staining for plakophilin 3 is seen in most desmosomes and partly dispersed in cytoplasm under various fixation conditions (FA, MeAc long, MeAc short):

Positive tissues:
- Stratified squamous epithelia: palmar, epidermis, scalp skin
- Stratified epithelia: tongue, vagina, esophagus
- Transitional epithelia: bladder
- Non-stratified (single layered) epithelia: colon, duodenum, pancreas, several glands
- Non-epithelial: reticulum cells of lymph node follicle

Negative tissues:
- Liver, heart, skeletal muscle, endothelia (vessels)

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