protag-HiRes anti-mScarlet-i-X2 Atto 647N
- Atto 647N conjugate
- Llama/alpaca single-domain antibody
- Suitable for ICC/IF
- Reacts with dsRed1, dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato
|Antibody Type||Recombinant, produced in E.coli|
|Reactivity||dsRed1/dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato|
|No reactivity||Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, mTagBFP or their most common derivatives|
|Storage||Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!|
|Intended use||Research use only|
|Formulation||2.5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:500|
protag-HiRes anti-mScarlet-i camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes mScarlet-i in its native conformation with high affinity and specificity. It can also recognize some mRFP-derived red fluorescent protein like mOrange2, dsRed, tdTomato, mRFP and mCherry in its native conformation. It does not cross-react with GFP or mTagBFP derivatives.
In protag-HiRes anti-mScarlet-i-X2, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. protag-HiRes anti-mScarlet-i-X2 can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, the protag-HiRes anti-mScarlet-i-X2 can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - render the protag-HiRes anti-mScarlet-i-X2 products superior tools for all microscopy techniques.
Q & A's
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Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.