Epitope mapped
Highly published
Diagnostic marker

anti-Vimentin mouse monoclonal, VIM 3B4, liquid, purified, sample

Liquid

Liquid

Lyophilized

FITC

Prediluted

Cat. No: 690013S

Available, delivery time 1-7 days

$96.00*

Key Features
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with amphibia, bovine, chicken, human, mouse and monkey
  • Isotype: IgG2a
Product description
Quantity 200 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Amphibia, Bovine, Chicken, Human, Monkey, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone VIM 3B4
Immunogen Vimentin purified from bovine lens
Concentration 50 µg/ml (10 µg)
Formulation PBS pH 7.4 with 0.09% sodium azide and 0.5% BSA
UniprotID P48616 (Bovine), P09654 (Chicken), P08670 (Human)
Synonym Vimentin, VIM
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:100-1:200 (250-500 ng/ml)
Immunohistochemistry (IHC) - paraffin 1:100-1:200 (250-500 ng/ml, protease treatment and/or microwave treatment recommended)
Western Blot (WB) 1:500-1:5,000 (10-100 ng/ml)
Background

The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).

Tested cultured cell lines: fibroblasts (SV-80).

Bohn W, Wiegers W, Beuttenmüller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992).
Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).

References/Publications (22)
Publication
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Application
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Category Protocol
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Category Protocol
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Positive western blot control: anti-Vimentin antibody
Cat. No: 64001
  • Whole cell lysate from SV80 human lung fibroblast (SV40-transformed) cell line
  • Validated and tested western blot control for: anti-Vimentin mouse monoclonal antibody, VIM 3B4, Cat. No. 61013

$85.00*
anti-Vimentin mouse monoclonal, XL-VIM-14.13, supernatant
Cat. No: 65189
  • Mouse monoclonal
  • Suitable for IEM, IHC and WB
  • Reacts with huamn, carp and trout
  • Isotype: IgG1

$245.00*
anti-Vimentin guinea pig polyclonal, serum
Cat. No: GP53
  • Guinea pig poplyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human and mouse

$306.00*
anti-Vimentin (N-terminus) guinea pig polyclonal, serum
Cat. No: GP59
  • Guinea pig poplyclonal
  • Suitable for IHC and WB
  • Reacts with human and mouse

$332.00*
anti-Vimentin (C-terminus) guinea pig polyclonal, serum
Cat. No: GP58
  • Guinea pig poplyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

$332.00*
anti-Vimentin guinea pig polyclonal, lyophilized, purified
Cat. No: 610130
  • Guinea pig polyclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human and mouse

$335.00*
Vimentin, bovine, 250 µg
Cat. No: 62011
Quantity: 250 µg
  • Purified bovine vimentin
  • Protein standard

Variants from $364.00*
$673.00*
Vimentin, human recombinant, 250 µg
Cat. No: 62015
Quantity: 250 µg
  • Recombinant human vimentin
  • Protein standard

Variants from $364.00*
$746.00*