Epitope mapped
Disease related

anti-Nucleolar Protein NO38 mouse monoclonal, No-63, ascites fluid

Supernatant

Ascites fluid

Cat. No: 612123

Available, delivery time 1-7 days

$271.00*

Key Features
  • Ascites fluid
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with chicken, pleurodelis, triturus and xenopus
  • Isotype: IgG2a
Product description
Quantity 250 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application IHC, IP, WB
Purification Ascites
Reactivity Chicken, Pleurodeles, Triturus, Xenopus
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone No-63
Immunogen Nucleolar fraction prepared from Xenopus laevis oocytes
Formulation Contains 0.09% sodium azide
UniprotID
Synonym Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:300-1:500 (also after fixation with paraformaldehyde)
Immunoprecipitation (IP) Assay dependent (from Xenopus oocyte extracts nucleoplasmin - a 30 kDa protein - is co-precipitated, which shows high sequence homology to NO38)
Western Blot (WB) 1:1,000-1:2,000 (for blotting transfer nitrocellulose sheets are superior to nylon sheets)
Background
Recognizes a 38 kDa nucleolar protein (NO38) in Xenopus (also termed numatrin, B23, nucleophosmin, in other species). No-63 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
References/Publications (3)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

anti-Nucleolar Protein NO38 mouse monoclonal, No-185, supernatant concentrate
Cat. No: 652125
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with chicken, pleurodelis, triturus and xenopus
  • Isotype: IgG1

$339.00*