Frequently used

anti-DNA mouse monoclonal, AC-30-10, lyophilized, purified

Cat. No: 61014

Available, delivery time 1-7 days

$281.00*

Key Features
  • Mouse monoclonal
  • Suitable for Dot Blots, ICC/IF and IHC
  • Reacts with all species
  • Isotype: IgM
Product description
Quantity 100 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgM
Conjugate Unconjugated
Application Dot blot, ICC/IF, IHC
Purification Size exclusion chromatography
Reactivity All species
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AC-30-10
Immunogen Double- and single-stranded DNA
Concentration 100 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Dot Blot Assay dependent (on nitrocellulose membrane, after baking at 70°C)
Background

AC-30-10 stains DNA present in the nuclei and the mitochondria of cells and tissues. It is also a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. Due to the high sensitivity it can also be used for the detection of mycoplasma contamination in cell cultures.
Binding characteristics: reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA.
Tested cultured cell lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.

References/Publications (20)
Publication
Species
Application
Species mouse,rat,rat,xenopus,Crithidia cuciliae,Triturus,chicken,bovine,M13 single and double stranded,recombinant plasmid DNA
Application IHC (frozen),IF,ICC-IF,IEM,dot blot
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