anti-Cytokeratin Type I & II, AE1/AE3, purified
- Mouse monoclonal
- Suitable for ICC/IF and IHC
- Reacts with bovine, chicken, guinea pig, human, monkey, mouse, rabbit and rat
- Isotype: IgG1
|Quantity||1 ml (200 µg/ml)|
|Reactivity||Bovine, Chicken, Guinea pig, Human, Monkey, Mouse, Rabbit, Rat|
|Intended use||Research use only|
|Immunogen||Human epidermal keratin|
|Formulation||0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:250-1:750|
|Immunohistochemistry (IHC) - paraffin||1:250-1:750 (protease treatment and/or microwave treatment recommended)|
AE1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors; reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins).
Polypeptides reacting: Mr 40 kDa, Mr 48 kDa and Mr 50 kDa polypeptides of human epithelial proteins (keratins nos. K19, K16, K14) and most other acidic (type I) keratins (AE1); most basic (type II) keratins (Mr 52.5-68 kDa) of human epithelia (AE3)
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Stabilized antiserum
- Guinea pig polyclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with human and mouse