Disease related
Highly published

anti-Cytokeratin Type I & II, AE1/AE3, purified

Cat. No: 61835

Available, delivery time 1-7 days

$442.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with bovine, chicken, guinea pig, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1
Product description
Quantity 1 ml (200 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC
Purification Affinity chromatography
Reactivity Bovine, Chicken, Guinea pig, Human, Monkey, Mouse, Rabbit, Rat
Intended use Research use only
Clone AE1/AE3
Immunogen Human epidermal keratin
Formulation 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:250-1:750
Immunohistochemistry (IHC) - paraffin 1:250-1:750 (protease treatment and/or microwave treatment recommended)
Background

AE1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors; reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins).
Polypeptides reacting: Mr 40 kDa, Mr 48 kDa and Mr 50 kDa polypeptides of human epithelial proteins (keratins nos. K19, K16, K14) and most other acidic (type I) keratins (AE1); most basic (type II) keratins (Mr 52.5-68 kDa) of human epithelia (AE3)

References/Publications (10)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
anti-Keratin Pan rabbit polyclonal, purified
Cat. No: 10550
  • Rabbit Polyclonal
  • Suitable for IHC on frozen and  paraffin sections
  • Reacts with human and mouse

$232.00*
anti-Keratin Pan guinea pig polyclonal, serum
Cat. No: GP14
Quantity: 100 µl
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

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