Diagnostic marker

anti-Desmoglein 2 mouse monoclonal, G129, supernatant

Supernatant

Sample

Cat. No: 651119

Available, delivery time 1-7 days

$269.00*

Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, rat
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone G129
Immunogen Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain)
Formulation Contains 0.09% sodium azide
UniprotID Q14126 (Human)
Synonym Desmoglein-2, Cadherin family member 5, HDGC, DSG2, CDHF5
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Ready-to-use (for better resolution preincubation (directliy after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended)
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) Assay dependent
Background

Antibody recognizes desmoglein 2 present in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.

Polypeptide reacting: Desmoglein 2 (1069aa; calculated mol. wt. 116,760).

Recommended as positive control: CaCo-2, HaCaT.

Reactivity on cultured cell lines: many, including CaCo-2, HaCaT, MCF-7.

References/Publications (2)
Downloads
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Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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