Disease related

anti-Desmoglein 3 mouse monoclonal, Dsg-G194, supernatant

Supernatant

Sample

Cat. No: 651112

Available, delivery time 1-7 days

$290.00*

Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Dsg-G194
Immunogen Recombinant human polypeptide Desmoglein 3 (C-terminal aa 920-940 VSIPDPLQHGNYLVTETYSAS)
Formulation Contains 0.09% sodium azide
UniprotID P32926 (Human)
Synonym Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6, DSG3, CDHF6
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Undiluted-1:50 (for better resolution preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended)
Immunohistochemistry (IHC) - paraffin Undiluted-1:50 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

In immunoblots of cytoskeletal preparations from human epidermis the antibody reacts specifically with a 140 kDa polypeptide typical for Desmoglein 3. In immunolocalization studies the mab shows typical staining of basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus vulgaris patient sera.
Cross-reaction with Desmogleins 1 and 2 was not detected.

References/Publications (1)
Downloads
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Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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