AAV8 ELISA Control

Cat. No: PRAAV8-C

Available, delivery time 1-7 days

$137.00*

Key Features
  • Consists of fully assembled, empty AAV8 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA
Product description
Quantity 1 vial (2.8E+08-4.7E+08 capsids)
Application ELISA
Storage 2-8°C
Intended use Research use only
Concentration

3.8E+08-6.3E+08 capsids/ml after reconstitution in 750 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial)

Formulation Lyophilized, empty AAV8 capsids. Reconstitute in 750 µl ASSB 1x (provided with PROGEN's AAV8 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red and ASSB 1x buffer.
Stability 4 weeks at 2-8°C after reconstitution in ASSB 1x
Applications
Tested applications Tested dilutions
ELISA As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN's AAV8 Titration ELISA) and analysis at least in duplicates is recommended.
Background

The AAV8 ELISA Control consists of fully assembled, empty AAV8 capsids. The concentration is lot specific.

The AAV8 ELISA Control can be used as a positive control with PROGEN's AAV8 Titration ELISA (PRAAV8). If you require different lots of the AAV8 ELISA Control and the Kit Control included in your PROGEN AAV8 Titration ELISA, please enquire to check availability.

Reading of the AAV8 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN's AAV8 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.

The AAV8 ELISA Control has been calibrated on the ATCC AAV8 reference standard material (RSM, VR-1816). For further information, please see the publication by Ayuso et al., 2014.

Publications

Ayuso, E. et al. Manufacturing and characterization of a recombinant adeno-associated virus type 8 reference standard material. Hum. Gene Ther. 25, 977–987 (2014).


References/Publications (2)
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FAQs
Yes, PROGEN offers serotype-specific AAV ELISA Controls for the standardization of your AAV titer determination. The serotype-specific positive controls are fully characterized empty AAV capsids standardized with PROGEN´s internal gold standards* (AAV1, AAV3, AAV5, AAV6 & AAV9) or the ATCC international gold standard material (AAV2 & AAV8).

*For more information on PROGEN´s internal gold standard, please check out our posters "Developing Reliable AAV Standards for ELISA" and "The New AAV3 Titration ELISA" describing the establishment and characterization of AAV5 and AAV3 internal gold standards.
AAV8 ELISA Control
Cat. No: PRAAV8-C
  • Consists of fully assembled, empty AAV8 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$137.00*
AAV8 empty capsids
Cat. No: 66V080
  • Fully assembled, empty AAV8 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

$300.00*
AAV8 Titration ELISA
Cat. No: PRAAV8
  • ELISA for quantitation of AAV8 capsids
  • Detection of full and empty AA8 capsids
  • ADK8 antibody is used as capture and detection antibody

$687.00*
AAV8 Xpress ELISA
Cat. No: PRAAV8XP
Fast, easy and reliable AAV8 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV8 Titration ELISA
  • ADK8 antibody is used as capture and detection antibody

$837.00*
AAVrh10 Titration ELISA
Cat. No: PRAAV10
  • ELISA for quantitation of AAVrh10 capsids
  • Detection of full and empty AAVrh10 capsids
  • ADK8 antibody is used as capture and detection antibody

$687.00*
anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified
Cat. No: 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

$468.00*
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
Cat. No: 690058
Quantity: 1 ml
  • Mouse monoclonal
  • Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
  • Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1

Variants from $130.00*
$457.00*
anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No: 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11 and AAV12

$468.00*
anti-AAV8 (intact particle) mouse monoclonal, ADK8, lyophilized, purified
Cat. No: 610160
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization
  • Reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
  • Isotype: IgG2a kappa

Variants from $133.00*
$445.00*
anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9, liquid, purified
Cat. No: 690161
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for dot blot and ICC/IF
  • Reacts with AAV8, AAV9, AAVrh10, AAVDJ, Anc80 and AAVrh74 intact particles
  • Isotype: IgG2a kappa

Variants from $133.00*
$445.00*
Dip’n’Check AAV8 - lateral flow assay
Cat. No: PR5208
  • Rapid lateral flow test for the detection of AAV8 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV8 capsids

$204.00*
OptiPrep™ (Iodixanol)
Cat. No: 1893 (previous 1114542)
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

Please note: The Cat. No. of OptiPrepTM changes from 1114542 to 1893 but the product itself has not been changed in any way.
Therefore it is possible to receive the ordered products with Cat. No. 1114542 or 1893.

$198.00*