AAV5 ELISA Control

Cat. No: PRAAV5-C

Available, delivery time 1-7 days

$137.00*

Key Features
  • Consists of fully assembled, empty AAV5 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA
Product description
Quantity 1 vial (3.9E+09-6.5E+09 capsids)
Application ELISA
Storage 2-8°C
Intended use Research use only
Concentration

7.9E+09-1.3E+10 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial)

Formulation Lyophilized, empty AAV5 capsids. Reconstitute in 500 µl ASSB 1x (provided with PROGEN's AAV5 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red and ASSB 1x buffer.
Stability 4 weeks at 2-8°C after reconstitution in ASSB 1x
Applications
Tested applications Tested dilutions
ELISA As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN's AAV5 Titration ELISA) and analysis at least in duplicates is recommended.
Background

The AAV5 ELISA Control consists of fully assembled, empty AAV5 capsids. The concentration is lot specific.

The AAV5 ELISA Control can be used as a positive control with PROGEN's AAV5 Titration ELISA (PRAAV5). If you require different lots of the AAV5 ELISA Control and the Kit Control included in your PROGEN AAV5 Titration ELISA, please enquire to check availability.

Reading of the AAV5 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN's AAV5 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.

The AAV5 ELISA Control has been calibrated on an internally established reference standard. The internal reference standard is a preparation of full AAV5 capsids that has been characterized by qPCR and ddPCR (DNA quantification) and electron microscopy (ratio of full to empty capsids). For further information, please see our poster Developing reliable AAV standards for ELISA available at aavvirus.com.

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FAQs
Yes, PROGEN offers serotype-specific AAV ELISA Controls for the standardization of your AAV titer determination. The serotype-specific positive controls are fully characterized empty AAV capsids standardized with PROGEN´s internal gold standards* (AAV1, AAV3, AAV5, AAV6 & AAV9) or the ATCC international gold standard material (AAV2 & AAV8).

*For more information on PROGEN´s internal gold standard, please check out our posters "Developing Reliable AAV Standards for ELISA" and "The New AAV3 Titration ELISA" describing the establishment and characterization of AAV5 and AAV3 internal gold standards.
AAV5 ELISA Control
Cat. No: PRAAV5-C
  • Consists of fully assembled, empty AAV5 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$137.00*
AAV5 empty capsids
Cat. No: 66V050
  • Fully assembled, empty AAV5 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

$300.00*
AAV5 Titration ELISA
Cat. No: PRAAV5
  • ELISA for quantitation of AAV5 capsids
  • Detection of full and empty AAV5 capsids
  • ADK5a antibody is used as capture and detection antibody

$687.00*
AAV5 Xpress ELISA
Cat. No: PRAAV5XP
Fast, easy and reliable AAV5 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV5 Titration ELISA
  • ADK5a antibody is used as capture and detection antibody

$837.00*
anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified
Cat. No: 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

$468.00*
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
Cat. No: 690058
Quantity: 1 ml
  • Mouse monoclonal
  • Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
  • Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1

Variants from $130.00*
$457.00*
anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No: 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11 and AAV12

$468.00*
anti-AAV5 (intact particle) mouse monoclonal, ADK5a, lyophilized, purified
Cat. No: 610148
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization assay
  • Reacts with AAV5 intact capsids
  • Isotype: IgG2a kappa

Variants from $133.00*
$445.00*
anti-AAV5 (intact particle) mouse monoclonal, ADK5b, lyophilized, purified
Cat. No: 610149
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, ICC and neutralization assays
  • Reacts with AAV5 intact capsids
  • Isotype: IgG2b kappa

Variants from $133.00*
$445.00*
anti-AAV5 rabbit polyclonal, serum
Cat. No: 610137
  • Rabbit polyclonal
  • Suitable for Affinity Chromatography, ICC/IF and WB
  • Reacts with AAV5

$476.00*
OptiPrep™ - Iodixanol
Cat. No: 1893 (previous 1114542)
  • Highly effective for the separation of full and empty AAV particles
  • Designed for the purification of viruses (such as AAV)
  • Useful to isolate different cell types and organelles
  • Ready-made, sterile and endotoxin-tested solution of iodixanol

$198.00*