Highly published

anti-Collagen Type IV rabbit polyclonal, serum

Cat. No: 10760

Available, delivery time 1-7 days

$293.00*

Key Features
  • Rabbit polyclonal
  • Suitable for IHC
  • Reacts with humans, mouse and rat
Product description
Quantity 250 µl
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC
Purification Stabilized antiserum
Reactivity Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Isolated from human placenta
Formulation Contains 0.09% sodium azide
UniprotID P02462 (Human),P02463 (Mouse),F1MA59 (Rat)
Synonym Collagen alpha-1(IV chain [Cleaved into: Arresten], COL4A1
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50 - 1:100
Immunohistochemistry (IHC) - paraffin 1:50 - 1:100 (proteolytic treatment required)
Background

The antibody is reactive with collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antiserum is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. In immunohistochemistry no cross-reactivity with other collagens at optimal dilutions. In immunoblotting, a slight cross-reactivity with collagen type V is observed.

Positive control: Skin, kidney.

References/Publications (8)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
anti-rabbit IgG goat polyclonal, HRP conjugate
Cat. No: 90003
  • HRP conjugate
  • Goat polyclonal
  • Suitable for ELISA and WB
  • Reacts with rabbit

$109.00*
anti-Collagen Type IV mouse monoclonal, 1042.0, supernatant
Cat. No: 10710
  • Mouse monoclonal
  • Suitable for IHC
  • Reacts with human
  • Isotype: IgG2b

$324.00*