AAV2 VP1, recombinant protein

Cat. No: 640823

Available, delivery time 1-10 days

$342.00*

Key Features
•    Recombinant AAV2 capsid protein VP1
•    Useful in combination with AAV2 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

Product description
Quantity 10 µg
Application Capillary electrophoresis (CE), Dot blot, SDS PAGE, WB
Purification Ni-NTA chromatography
Storage -80°C
Intended use Research use only
Concentration 100 µg/ml (1.19 µM)
Formulation Liquid, 6 M urea in PBS
Source Escherichia coli
Molecular weight ~90 kDa
Purity > 90% (determined by SDS PAGE)
Product description N-terminal His-tagged recombinant AAV2 capsid protein VP1
UniprotID P03135 (AAV2)
Applications
Tested applications Tested dilutions
Capillary electrophoresis (CE) Assay dependent
Dot Blot 100 ng, depending on primary antibody and detection method
SDS PAGE 1 µg
Western Blot (WB) 5-20 ng, depending on primary antibody and detection method
Background

The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
The recombinant AAV2 VP1 protein in combination with recombinant AAV2 VP2 (Cat. No. 640824) and recombinant AAV2 VP3 (Cat. No. 640825) can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot.
All three recombinant AAV2 capsid proteins are available as set (Cat. No. 72001) or as individual proteins (Cat. No. 640823, 640824, 640825).

Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
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AAV2 ELISA Control
Cat. No: PRAAV2-C
  • Consists of fully assembled, empty AAV2 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$137.00*
AAV2 empty capsids
Cat. No: 66V020
  • Fully assembled, empty AAV2 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

$300.00*
AAV2 Titration ELISA
Cat. No: PRATV
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20 antibody is used as capture and detection antibody

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AAV2 Titration ELISA 2.0R
Cat. No: PRAAV2R
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20R antibody is used as capture and detection antibody

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AAV2 VP1 + VP2 + VP3, recombinant proteins, set
Cat. No: 72001
•    Recombinant AAV2 VP1-3 capsid protein set
•    Useful for ratio analysis
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.


$842.00*
AAV2 VP1, recombinant protein
Cat. No: 640823
•    Recombinant AAV2 capsid protein VP1
•    Useful in combination with AAV2 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

$342.00*
AAV2 VP2, recombinant protein
Cat. No: 640824
•    Recombinant AAV2 capsid protein VP2
•    Useful in combination with AAV2 VP1 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.


$342.00*
AAV2 VP3, recombinant protein
Cat. No: 640825
•    Recombinant AAV2 capsid protein VP3
•    Useful in combination with AAV2 VP1 and VP2 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.



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AAV2 Xpress ELISA
Cat. No: PRAAV2XP
Fast, easy and reliable AAV2 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV2 Titration ELISAs
  • A20R antibody is used as capture and detection antibody

$837.00*
anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified
Cat. No: 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

$468.00*
anti-AAV VP1/VP2 mouse monoclonal, A69, lyophilized, purified
Cat. No: 61057
  • Mouse monoclonal
  • Suitable for ICC/IF, IP and WB
  • Reacts with AAV2 and AAVDJ
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$445.00*
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
Cat. No: 690058
Quantity: 1 ml
  • Mouse monoclonal
  • Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
  • Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1

Variants from $130.00*
$457.00*
anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No: 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11 and AAV12

$468.00*
anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified
Cat. No: 61055
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA, ICC and neutralization assay
  • Reacts with AAV2, AAV2 7m8 and AAV3 intact particles
  • Isotype: IgG3

Variants from $133.00*
$468.00*
anti-AAV2 (intact particle) mouse recombinant, A20R, lyophilized, purified
Cat. No: 610298
Quantity: 50 µg
  • Purified, lyophilized
  • Recombinant antibody
  • Suitable for dot blot, ELISA, neutralization assay
  • Reacts with AAV2, AAV3, AAV2 7m8 and Anc80 intact particles
  • Isotype: mouse IgG1

Variants from $133.00*
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anti-AAV2 Replicase mouse monoclonal, 226.7, supernatant
Cat. No: 65172
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  • Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
  • Isotype IgG1

$269.00*
anti-AAV2 Replicase mouse monoclonal, 259.5, lyophilized, purified
Cat. No: 61071
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
  • Isotype: IgG1

Variants from $269.00*
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anti-AAV2 Replicase mouse monoclonal, 303.9, lyophilized, purified
Cat. No: 61069
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
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$476.00*
anti-AAV2 Replicase mouse monoclonal, 76.3, lyophilized, purified
Cat. No: 61073
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  • Suitable for WB, ICC/IF and IP
  • Reacts with AAV2 Rep52 and Rep78
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Dip’n’Check AAV2 and AAV3
Cat. No: PR5223
  • Rapid lateral flow test for the detection of AAV2 and AAV3 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV2 and AAV3 capsids

$204.00*
OptiPrep™ (Iodixanol)
Cat. No: 1893 (previous 1114542)
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

Please note: The Cat. No. of OptiPrepTM changes from 1114542 to 1893 but the product itself has not been changed in any way.
Therefore it is possible to receive the ordered products with Cat. No. 1114542 or 1893.

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Simple Plex AAV2 Assay
Cat. No: SPCKB-OT-005905
  • Automated ELISA kit for quantification of AAV2 virons and assembled capsids
  • A20R antibody (Cat. No. 610298) is used as capture and detection antibody
  • Suitable for the ProteinSimple Ella system
Product is not available in APAC countries

$950.00*