anti-Biotin mouse monoclonal, Hyb-8, purified

Cat. No: 691698

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF, IHC and WB
  • Reacts with biotin
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Biotin
Storage 2-8°C
Intended use Research use only
Clone Hyb-8
Immunogen Biotinylated sheep immunoglobulin while NHS-biotin was used as a biotinylation agent
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Western Blot (WB) 1:100-1:200 (0.5-1 µg/ml)
Background

Hyb-8 recognizes free biotin and biotinylated proteins (HS biotin, NHS amidocaproylbiotin). The length of the spacer does not affect mAb binding. The antibody also react with biotinylated DNA probes.

Positive Control: Tissues enriched with biotinylated probes.

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