Epitope mapped

anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2, FITC Conjugate

anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2, FITC Conjugate View more

Lyophilized

100 µg

Lyophilized

50 µg

HRP

1 ml (100 µg/ml)

HRP

250 µl (100 µg/ml)

Biotin

250 µl (100 µg/ml)

FITC

250 µl (400 µg/ml)

Cat. No: 61497

Prices excl. tax

Available, delivery time 1-7 days

$531.00*

Datasheet

Key Features

FITC conjugate
Mouse monoclonal
Suitable for phage display
Reacts with filamentous phages F1, filamentous phages fd and filamentous phages M13
Isotype: IgG2b

Product description

Quantity	250 µl (400 µg/ml)
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG2b
Conjugate	FITC
Application	Phage display
Purification	Affinity chromatography
Reactivity	Filamentous phages F1, Filamentous phages M13, Filamentous phages fd
Storage	2-8°C
Intended use	Research use only
Clone	B62-FE2
Immunogen	Fd phages from E. coli F+ strain (JM109)
Concentration	400 µg/ml
Formulation	Tris buffer (pH 8.0), 0.5% BSA, 0.09% sodium azide
Synonym	M13
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Phage Display	1 µg/ml; immunoassays for the identification of recombinant antigen- or antibody-phages; Detection limit: 1E+07 phage particles

Background

B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (Kneissel et al. 1999).

Kneissel, S. et al. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-8 (1999).

References/Publications (4)

Publication

Species

Application

Publication Seidel-Greven, M. et al. Isolation and light chain shuffling of a Plasmodium falciparum AMA1-specific human monoclonal antibody with growth inhibitory activity. Malar. J. 20, 1–20 (2021).

Species M13

Application ELISA

Publication Rondot, S., Koch, J., Breitling, F. & Dübel, S. A helper phage to improve single-chain antibody presentation in phage display. Nat. Biotechnol. 19, 75-8 (2001).

Species M13

Application ELISA

Publication Kneissel, S. et al. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-8 (1999).

Species fd

Application WB

Publication Micheel, B. et al. Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages. J. Immunol. Methods 171, 103-9 (1994).

Species fd

Application ELISA,IEM

Downloads

Datasheet

Q & A's

Please enter a valid email address.

Your question *

Please enter a valid question.

I agree that my data will be processed to answer the question. I have taken note of the Privacy Policy and agree to the information described therein.*

Fields marked with * are mandatory.

There aren't any asked questions yet.

Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.

Login

Your email address

Your password

Display reviews in current language only.

No reviews found. Be the first to share your insights.

FAQs

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.

PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:

Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Progen Support

For scientific and customer support, you can reach us by phone or email.