Disease related
Highly published

anti-Keratin Type I mouse monoclonal, AE1, purified

Cat. No: 61804

Available, delivery time 1-7 days

$271.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF,  IHC and WB
  • Reacts with bovine, chicken, dog, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1
Product description
Quantity 200 µg (1 mg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Chicken, Dog, Human, Monkey, Mouse, Rabbit, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AE1
Immunogen Human epidermal keratin (formerly also designated cytokeratin)
Concentration 1 mg/ml
Formulation PBS pH 7.4 with 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:200 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

AE 1 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: all epithelium-derived tumors.
Polypeptide reacting: Mr 40,000; Mr 48,000 and Mr 50,000 polypeptides of human epithelial proteins (keratins nos. K19, K16, K14) and most other acidic (type I) keratins.
Reactivity on cultured cell lines: MCF-7, A-431, RT 112.

References/Publications (15)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
anti-Keratin Type II mouse monoclonal, Ks pan1-8, lyophilized, purified
Cat. No: 61006
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with amphibia, bovine, human, mouse and rat
  • Isotype: IgG2a

$251.00*
anti-Keratin Type II mouse monoclonal, AE3, purified
Cat. No: 61806
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, chicken, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1

$271.00*