anti-Keratin K15 guinea pig polyclonal, serum

Cat. No: GP-CK15

Available, delivery time 1-7 days

$311.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human and mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen C-terminal tail region (including parts of the rod domain) of human recombinant keratin K15
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID P19012 (Human),Q61414 (Mouse)
Synonym Keratin, type I cytoskeletal 15, Cytokeratin-15, CK-15, Keratin-15, K15, KRT15, KRTB
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100-1:200
Western Blot (WB) 1:2,000
Background

Mr 50 kDa (pI 4.9) intermediate filament polypeptide, keratin K15 (formerly also designated cytokeratin 15), detected by immunofluorescence microscopy in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. broncheal epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the outermost cell layer of the outer root sheath; within the hair follicle several cell layers of the hair follicle bulb were positive with antiserum gp 15.1.

In human fetal tissues keratin K15 was localized in basal cells of epidermis, lung, trachea, tracheal glands and esophagus. In fetal hair anlagen, all cells were positive with antiserum gp 15.1, suggesting that keratin K15 is preferentially expressed in epithelial stem cells. Completely unreactive were cells of liver, colon, kidney, urinary bladder, pancreas and lung alveolus.

In tumors, gp15.1 is reactive with squamous skin and lung carcinomas and with a significant subgroup of invasive ductal breast carcinomas, especially tumors with higher grade malignancy.

Reactivity on cultured cell lines: HaCat, A-431; negative on PLC.

References/Publications (1)
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FAQs
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
anti-guinea pig IgG goat polyclonal, HRP conjugate
Cat. No: 90001
  • Goat polyclonal
  • Suitable for ELISA and WB
  • Reacts with guinea pig

$109.00*
anti-Keratin K15 guinea pig polyclonal, serum
Cat. No: GP-K15
  • Guinea pig polyclonal
  • Suitable for IHC
  • Reacts with human and mouse

$311.00*