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AAV2 VP3, recombinant protein

PROGEN Biotechnik GmbH
AAV2 VP3, recombinant protein
Western blot analysis of recombinant AAV2 VP3 protein (Cat. No. 640825) with fluorescent B1 antibody. Western blot analysis was performed on different amounts of recombinant AAV-VP3 ranging from 50 ng to 200 ng. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, AFDye™ 488 Conjugate (Cat. No. 61058-488) was diluted in blocking buffer (antibody concentration 0.13 µg/ml) and incubated for 1 h at RT. The bands were visualized by fluorescent detection.
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AAV2 VP3, recombinant protein
Western blot analysis of recombinant AAV2 capsid proteins (Cat. No. 640823, 640824, 640825) and denatured AAV2 cpasids with B1 antibody (Cat. No. 690058). Western blot analysis was performed on 3.3E+09 denatured AAV2 capsids and recombinant AAV2 VP proteins (ratio 1:1:10 - VP1:VP2:VP3). The PVDF membranes were blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT than probed with 0.2 µg/ml (AAV2 capsids) or 0.5 µg/ml (recombinant VP proteins) anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 as primary antibody diluted in blocking buffer for 1 h at 4°C. The secondary antibody goat anti-mouse IgG HRP was also diluted in blocking buffer (antibody concentration 0.2 µg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
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AAV2 VP3, recombinant protein
Pipetting scheme for western blot analysis using a mix of the AAV2 capsid proteins (Cat. No. 640823, 640824, 640825). To create a VP mixture with the molar ratio 1:1:10 (VP1:VP2:VP3), please pre-dilute VP1 and VP2 1:10 to yield a final concentration of 10 µg/ml (green table). Pipette the pre-diluted VP1 and VP2 proteins and mix them with the undiluted VP3 protein in your sample buffer and water (blue table). The example with 2x and 3x sample buffer and the required volumes are indicated in the pipetting scheme. Thus, in one lane, 10 µl of the VP mix can be loaded onto the SDS PAGE and analyzed by Western blot using the B1 antibody (Cat. No. 690058, Cat. No. 61058-488, Cat. No. 61058-647).
Undiluted = 100 µg/ml, pre-diluted = 10 µg/ml


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AAV2 VP3, recombinant protein
Pipetting scheme for western blot analysis using the AAV2 capsid proteins (Cat. No. 640823, 640824, 640825) in separate lanes. To analyze the molar ratio of 1:1:10, it is recommended to load VP1, VP2 and VP3 as described in the pipetting scheme above. Therefore, the indicated volumes of the proteins (concentration 100 µg/ml) should be diluted with the appropriate amount of sample buffer and distilled water.
10 µl of each solution can be separately loaded onto the SDS PAGE and analyzed by Western blot using the B1 antibody (Cat. No. 690058, Cat. No. 61058-488, Cat. No. 61058-647).


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AAV2 VP3, recombinant protein
SDS PAGE analysis to evaluate the purity of the AAV2 VP3 (Cat. No. 640825). To perform SDS PAGE analysis, 1 ug of AAV2 VP2 was diluted in 10 ul PBS and sample buffer and denatured at 95°C for 10 min. The sample was loaded onto a 4-20% gradient gel (40 min at 200 V). Afterwards, the gel was stained for 1 h at RT with Coomassie solution and destained with water. View more
AAV2 VP3, recombinant protein
Western blot analysis of recombinant AAV2 VP3 (Cat. No. 640825) with B1 antibody. Western blot analysis was performed on different amounts of recombinant AAV-VP3 ranging from 25 ng to 100 ng. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 (Cat. No. 690058) was diluted in blocking buffer (antibody concentration 0.5 µg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG HRP was also diluted in blocking buffer (antibody concentration 0.2 µg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
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AAV2 VP3, recombinant protein
AAV2 VP3, recombinant protein
AAV2 VP3, recombinant protein
AAV2 VP3, recombinant protein
AAV2 VP3, recombinant protein
AAV2 VP3, recombinant protein
Cat. No: 640825

Prices excl. VAT and shipping costs

Available, delivery time 1-10 days

$342.00*

Datasheet
Key Features
•    Recombinant AAV2 capsid protein VP3
•    Useful in combination with AAV2 VP1 and VP2 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.



Product description
Quantity 10 µg
Application Capillary electrophoresis (CE), Dot blot, SDS PAGE, WB
Purification Ni-NTA chromatography
Storage -80°C
Intended use Research use only
Concentration 100 µg/ml (1.61 µM)
Formulation Liquid, 6 M urea in PBS
Source Escherichia coli
Molecular weight ~62 kDa
Purity > 90% (determined by SDS PAGE)
Product description N-terminal His-tagged recombinant AAV2 capsid protein VP3
Applications
Tested applications Tested dilutions
Capillary electrophoresis (CE) Assay dependent
Dot Blot 100 ng, depending on primary antibody and detection method
SDS PAGE 1 µg
Western Blot (WB) 5-20 ng, depending on primary antibody and detection method
Background

The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
The recombinant AAV2 VP3 protein in combination with recombinant AAV2 VP1 (Cat. No. 640823) and recombinant AAV2 VP2 (Cat. No. 640824) can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot.
All three recombinant AAV2 capsid proteins are available as set (Cat. No. 72001) or as individual proteins (Cat. No. 640823, 640824, 640825).

Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
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File WB protocol
Category Protocol
Size 919.99 KB
Filetype pdf
Datasheet
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AAV Standards


AAV ELISA


AAV Antibodies

AAV2 ELISA Control
AAV2 ELISA Control
Cat. No: PRAAV2-C
  • Consists of fully assembled, empty AAV2 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$137.00*
AAV2 empty capsids
AAV2 empty capsids
Cat. No: 66V020
  • Fully assembled, empty AAV2 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

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AAV2 Titration ELISA
AAV2 Titration ELISA
Cat. No: PRATV
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20 antibody is used as capture and detection antibody

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AAV2 Titration ELISA 2.0R
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Cat. No: PRAAV2R
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20R antibody is used as capture and detection antibody

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AAV2 VP1 + VP2 + VP3, recombinant proteins, set
AAV2 VP1 + VP2 + VP3, recombinant proteins, set
Cat. No: 72001
•    Recombinant AAV2 VP1-3 capsid protein set
•    Useful for ratio analysis
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.


$842.00*
AAV2 VP1, recombinant protein
AAV2 VP1, recombinant protein
Cat. No: 640823
•    Recombinant AAV2 capsid protein VP1
•    Useful in combination with AAV2 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

$342.00*
AAV2 VP2, recombinant protein
AAV2 VP2, recombinant protein
Cat. No: 640824
•    Recombinant AAV2 capsid protein VP2
•    Useful in combination with AAV2 VP1 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.


$342.00*
AAV2 VP3, recombinant protein
AAV2 VP3, recombinant protein
Cat. No: 640825
•    Recombinant AAV2 capsid protein VP3
•    Useful in combination with AAV2 VP1 and VP2 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.



$342.00*
AAV2 Xpress ELISA
AAV2 Xpress ELISA
Cat. No: PRAAV2XP
Fast, easy and reliable AAV2 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV2 Titration ELISAs
  • A20R antibody is used as capture and detection antibody

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anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified
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Cat. No: 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
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anti-AAV VP1/VP2 mouse monoclonal, A69, lyophilized, purified
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Cat. No: 61057
  • Mouse monoclonal
  • Suitable for ICC/IF, IP and WB
  • Reacts with AAV2 and AAVDJ
  • Isotype: IgG1

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anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
Cat. No: 690058
Quantity: 1 ml
  • Mouse monoclonal
  • Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
  • Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1

Variants from $130.00*
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anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No: 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11 and AAV12

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anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified
anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified
Cat. No: 61055
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA, ICC and neutralization assay
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Variants from $133.00*
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Quantity: 50 µg
  • Purified, lyophilized
  • Recombinant antibody
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  • Reacts with AAV2, AAV3, AAV2 7m8 and Anc80 intact particles
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Variants from $133.00*
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anti-AAV2 Replicase mouse monoclonal, 226.7, supernatant
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Cat. No: 61071
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
  • Isotype: IgG1

Variants from $269.00*
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anti-AAV2 Replicase mouse monoclonal, 303.9, lyophilized, purified
anti-AAV2 Replicase mouse monoclonal, 303.9, lyophilized, purified
Cat. No: 61069
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  • Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
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anti-AAV2 Replicase mouse monoclonal, 76.3, lyophilized, purified
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  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
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  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

Please note: The Cat. No. of OptiPrepTM changes from 1114542 to 1893 but the product itself has not been changed in any way.
Therefore it is possible to receive the ordered products with Cat. No. 1114542 or 1893.

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