Disease related
Frequently used

anti-Desmoglein 1 mouse monoclonal, Dsg1-P124, supernatant

Supernatant

Sample

Cat. No: 651111

Available, delivery time 1-7 days

$290.00*

Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG2a
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Dsg1-P124
Immunogen Recombinant human Desmoglein 1 (extracellular part EII, EIII and EIV)
Formulation Contains 0.09% sodium azide
UniprotID Q02413 (Human)
Synonym Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen, DSG1, CDHF4
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Undiluted-1:50 (preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, recommended)
Immunohistochemistry (IHC) - paraffin Undiluted-1:50 (preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media and microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

In immunoblots of cytoskeletal preparations from human skin the antibody reacts specifically with a 160 kDa polypeptide typical for Desmoglein 1. In immunolocalization studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. Cross-reaction with Desmogleins 2 and 3 was not detected.

References/Publications (6)
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Category Protocol
Size 924.86 KB
Filetype pdf
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