Disease related
Highly published

anti-Desmoglein 1 mouse monoclonal, Dsg1-P124, supernatant

Supernatant

Sample

Cat. No: 651111

Available, delivery time 1-7 days

$290.00*

Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG2a
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Dsg1-P124
Immunogen Recombinant human Desmoglein 1 (extracellular part EII, EIII and EIV)
Formulation Contains 0.09% sodium azide
UniprotID Q02413 (Human)
Synonym Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen, DSG1, CDHF4
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Undiluted-1:50 (preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, recommended)
Immunohistochemistry (IHC) - paraffin Undiluted-1:50 (preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media and microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

In immunoblots of cytoskeletal preparations from human skin the antibody reacts specifically with a 160 kDa polypeptide typical for Desmoglein 1. In immunolocalization studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. Cross-reaction with Desmogleins 2 and 3 was not detected.

References/Publications (6)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

anti-Desmoglein 2 mouse monoclonal, G129, supernatant
Cat. No: 651119
Quantity: 5 ml
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, rat
  • Isotype: IgG1

Variants from $65.00*
$269.00*
anti-Desmoglein 2 mouse monoclonal, G96, supernatant
Cat. No: 651118
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human
  • Isotype: IgG1

$269.00*
anti-Desmoglein 3 mouse monoclonal, Dsg-G194, supernatant
Cat. No: 651112
Quantity: 5 ml
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG1

Variants from $65.00*
$290.00*
anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, lyophilized, purified
Cat. No: 61002
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, rat
  • Isotype: IgG1

Variants from $65.00*
$318.00*
anti-Desmoglein 2 mouse monoclonal, 10G11, lyophilized, purified
Cat. No: 61059
  • Mouse monclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human
  • Isotype: IgG1

$318.00*
anti-Desmoglein 2 rabbit polyclonal, serum
Cat. No: 610121
  • Rabbit polyclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human and mouse

$320.00*
anti-Desmoglein 4 guinea pig polyclonal, serum
Cat. No: GP4
Quantity: 100 µl
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for IHC
  • Reacts with human, mouse and rat

Variants from $65.00*
$366.00*
anti-Desmoglein 1 mouse monoclonal, Dsg1-P23, supernatant concentrate
Cat. No: 652110
Quantity: 500 µl
  • Hybridoma cell culture supernatant concentrate
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG1

Variants from $290.00*
$510.00*