AAV1 ELISA Control
- Consists of fully assembled, empty AAV1 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
Product description
| Quantity | 1 vial (0.6E+09-1.0E+09 capsids) |
|---|---|
| Application | ELISA |
| Storage | 2-8°C |
| Intended use | Research use only |
| Concentration | 1.2E+09-2.0E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial) |
| Formulation | Lyophilized, empty AAV1 capsids. Reconstitute in 500 µl ASSB 1x (provided with PROGEN's AAV1 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red and ASSB 1x buffer. |
| Stability | 4 weeks at 2-8°C after reconstitution in ASSB 1x |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN's AAV1 Titration ELISA) and analysis at least in duplicates is recommended. |
Background
The AAV1 ELISA Control consists of fully assembled, empty AAV1 capsids. The concentration is lot specific.
The AAV1 ELISA Control can be used as a positive control with PROGEN's AAV1 Titration ELISA (PRAAV1). If you require different lots of the AAV1 ELISA Control and the Kit Control included in your PROGEN AAV1 Titration ELISA, please enquire to check availability.
Reading of the AAV1 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN's AAV1 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.
The AAV1 ELISA Control has been calibrated on an internally established reference standard. The internal reference standard is a preparation of full AAV1 capsids that has been characterized by qPCR (DNA quantification) and electron microscopy (ratio of full to empty capsids). For further information, please see as an example our poster The New AAV3 Titration ELISA.
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FAQs
Empty capsids (AAV1, 2, 5, 6, 8, 9, rh10 and rh74) are provided in liquid format in PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl without protein additives. The titer is greater than 5.0E+12 capsids/ml. Therefore, they can be used in several applications.
ELISA Kit Controls (AAV1, 2, 3, 5, 6, 8, 9, rh10 and rh74) are lyophilized. After reconstitution in ASSB 1x the final solution contains stabilizing protein, phenol red and ASSB 1x buffer. The titer is lot-specific around 7.9E+09-1.3E+10 capsids/ml. This product is mainly suitable for the ELISA.
Yes, PROGEN offers serotype-specific AAV ELISA Controls for the standardization of your AAV titer determination.
The serotype-specific positive controls are fully characterized empty AAV capsids standardized with PROGEN´s internal gold standards* (AAV1, AAV3, AAV5, AAV6, AAV9, AAVrh10 and AAVrh74) or the ATCC international gold standard material (AAV2 & AAV8).
*For more information on PROGEN´s internal gold standard, take a look at our posters "Developing Reliable AAV Standards for ELISA" and "The New AAV3 Titration ELISA" describing the establishment and characterization of AAV5 and AAV3 internal gold standards.
Related Products
- Consists of fully assembled, empty AAV1 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
- Fully assembled, empty AAV1 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Fully assembled AAV1 capsids filled with an eGFP reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- ELISA for quantitation of AAV1 capsids
- Detection of full and empty AAV1 capsids
- ADK1a antibody is used as capture and detection antibody
- Mouse monoclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
- Isotype: IgG2a
- Mouse monoclonal
- Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
- Reacts with denatured VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
- Isotype: IgG1
- Rabbit polyclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
- Strong affinity for denatured capsid proteins
- Weak affinity for native capsids
- Purified, lyophilized
- Mouse monoclonal
- Suitable for dot blot, ELISA, ICC/IF and neutralization assay
- Reacts with AAV1, AAV6, AAV12 intact capsids
- Isotype: IgG2a lambda
- Recombinant chimeric (human Fc region)
- Suitable for dot blot, neutralization assay and serology ELISA
- Reacts with AAV1 and AAV6 intact capsids
- Isotype: IgG1
- Rapid lateral flow test for the detection of AAV1 and AAV6 capsids
- Semi-quantitative AAV titer determination in cell lysates and purified preparations
- Detection of full and empty AAV1 and AAV6 capsids
- Highly effective density gradient media for the separation of full and empty AAV particles
- Designed for the purification of viruses (such as AAV)
- Useful to isolate different cell types and organelles
- Ready-made, sterile and endotoxin-tested solution of iodixanol
- Automated ELISA kit for quantification of AAV1 virons and assembled capsids
- ADK1a antibody (Cat. No. 610150) is used as capture and detection antibody
- Suitable for the ProteinSimple Ella system