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AAV2 VP1, recombinant protein

Key Features
•    Recombinant AAV2 capsid protein VP1
•    Useful in combination with AAV2 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

Product description
Quantity 10 µg
Application Capillary electrophoresis (CE), Dot blot, SDS PAGE, WB
Purification Ni-NTA chromatography
Storage -80°C
Intended use Research use only
Concentration 100 µg/ml (1.19 µM)
Formulation Liquid, 6 M urea in PBS
Source Escherichia coli
Molecular weight 84.1 kDa (calculated Mw from aa sequence)
Purity > 90% (determined by SDS PAGE)
Product description

N-terminal His-tagged (MGSSHHHHHHSSGLVPRGSH) recombinant AAV2 capsid protein VP1

UniprotID P03135 (AAV2)
Applications
Tested applications Tested dilutions
Capillary electrophoresis (CE) Assay dependent
Dot Blot 100 ng, depending on primary antibody and detection method
SDS PAGE 1 µg
Western Blot (WB) 5-20 ng, depending on primary antibody and detection method
Background

The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
The recombinant AAV2 VP1 protein in combination with recombinant AAV2 VP2 (Cat. No. 640824) and recombinant AAV2 VP3 (Cat. No. 640825) can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot.
All three recombinant AAV2 capsid proteins are available as set (Cat. No. 72001) or as individual proteins (Cat. No. 640823, 640824, 640825).

Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
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Category Protocol
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Category Poster
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