AAV8 empty capsids

Cat. No: 66V080

Available, delivery time 1-7 days

$300.00*

Key Features
  • Fully assembled, empty AAV8 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
Quantity 100 µl (> 5.0E+11 capsids)
Application Dot blot, ELISA, WB
Purification Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific)
Storage Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid freeze/thaw cycles
Intended use Research use only
Concentration > 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial
Formulation PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl
Source Produced in HEK293T cells
Purity > 95% by SDS-PAGE and silver stain
Quality check Final titer was assigned on internal reference material using two AAV8 ELISA kits (PRAAV8 and PRAAV8XP); QC included analysis of filling grade by analytical ultra centrifugation (AUC) and Endotoxin testing
Product description Purified empty adeno-associated virus 8 capsids (AAV8)
Endotoxin < 1.0 EU/ml
Vector pRep2-Cap8 + pHelper
Note Please centrifuge before opening to ensure complete recovery of vial contents
Applications
Tested applications Tested dilutions
Dot Blot Depending on primary antibody and detection method
ELISA As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s AAV8 ELISA) and analysis at least in duplicates is recommended
Western Blot (WB) Depending on primary antibody and detection method
Background
Our AAV8 empty capsids consist of fully assembled, empty AAV8 capsids and have a titer of > 5.0E+12 viral particles/ml (vp/ml). Final concentrations are lot-specific and can be found on the corresponding vial.

The AAV8 empty capsids are provided with titers above 5.0E+12 viral particles/ml in a liquid formulation. Since the buffer does not contain any stabilizing proteins or dyes, the capsids can be used in various applications, including dot blot, western blot and ELISA. The lot-specific titers were assigned using two different AAV8 ELISA (PRAAV8 and PRAAV8XP). Internal standard material calibrated with the ATCC reference standard material for AAV8 (VR-1816) was used for the final titer declaration.
Our comprehensive quality control ensures well-characterized capsid material which can be implemented as reference material in a variety of assays to prove the validity of the corresponding assay.

PROGEN also provides empty capsid material for the AAV serotypes 1, 2, 5, 6 and 9.

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AAV8 ELISA Control
Cat. No: PRAAV8-C
  • Consists of fully assembled, empty AAV8 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$137.00*
AAV8 empty capsids
Cat. No: 66V080
  • Fully assembled, empty AAV8 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

$300.00*
AAV8 Titration ELISA
Cat. No: PRAAV8
  • ELISA for quantitation of AAV8 capsids
  • Detection of full and empty AA8 capsids
  • ADK8 antibody is used as capture and detection antibody

$687.00*
AAV8 Xpress ELISA
Cat. No: PRAAV8XP
Fast, easy and reliable AAV8 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV8 Titration ELISA
  • ADK8 antibody is used as capture and detection antibody

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AAVrh10 Titration ELISA
Cat. No: PRAAV10
  • ELISA for quantitation of AAVrh10 capsids
  • Detection of full and empty AAVrh10 capsids
  • ADK8 antibody is used as capture and detection antibody

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Cat. No: 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

$468.00*
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
Cat. No: 690058
Quantity: 1 ml
  • Mouse monoclonal
  • Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
  • Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
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Variants from $130.00*
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anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No: 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
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anti-AAV8 (intact particle) mouse monoclonal, ADK8, lyophilized, purified
Cat. No: 610160
Quantity: 50 µg
  • Purified, lyophilized
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  • Suitable for dot blot, ELISA and neutralization
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Variants from $133.00*
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Cat. No: 690161
Quantity: 1 ml (50 µg/ml)
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  • Mouse monoclonal
  • Suitable for dot blot and ICC/IF
  • Reacts with AAV8, AAV9, AAVrh10, AAVDJ, Anc80 and AAVrh74 intact particles
  • Isotype: IgG2a kappa

Variants from $133.00*
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Dip’n’Check AAV8
Cat. No: PR5208
  • Rapid lateral flow test for the detection of AAV8 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV8 capsids

$204.00*
OptiPrep™ (Iodixanol)
Cat. No: 1893 (previous 1114542)
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  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

Please note: The Cat. No. of OptiPrepTM changes from 1114542 to 1893 but the product itself has not been changed in any way.
Therefore it is possible to receive the ordered products with Cat. No. 1114542 or 1893.

$198.00*