- Fully assembled AAV2 capsids filled with an eGFP reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
| Filling grade | > 70% full capsids, please find the lot-specific filling grade on the CoA |
|---|---|
| Quantity | 100 µl |
| Application | Cell-based assay, ELISA, Neutralization assay, qPCR |
| Purification | Affinity Chromatography (POROS CaptureSelect AAV9 Affinity Resin, Thermo Fisher Scientific), Iodixanol gradient centrifugation for removal of empty capsids using OptiPrep (Cat. No. 1893, PROGEN) |
| Storage | Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles |
| Intended use | Research use only |
| Concentration | > 1.0E+12 vg/ml; please find the lot-specific concentration (total capsid and viral genome titer) on the CoA |
| Formulation | PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl |
| Source | Produced in HEK293T cells |
| Purity | > 95% by SDS-PAGE and silver stain |
| Quality check | Total capsid titer was assigned using AAV2 ELISA kits (PRAAV2R and PRAAV2XP, calibrated with ATCC reference standard material) and viral genome titer by qPCR using eGFP primers; QC included analysis of filling grade, purity, aggregation, and endotoxin testing. |
| Transfer Vector | pAAV-CMV-eGFP-WPRE |
| Packaging Plasmid | pDP2rs |
| Endotoxin | < 1.0 EU/ml (detection limit 1.0 EU/ml) |
| Note | Please centrifuge before opening to ensure complete recovery of vial contents; aliquoting and repeated freeze/thaw cycles can lead to a drop in titer |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Dot Blot | Depending on primary antibody and detection method |
| ELISA | As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s AAV2 ELISA), analysis at least in duplicates is recommended |
| Cell-based Assay | Depending on the experimental setup |
| PCR | As standard or positive control in qPCR or ddPCR - concentration depending on experimental setup |
| Neutralization Assay | Depending on the experimental setup |
| Western Blot (WB) | Depending on primary antibody and detection method |
Background
Our AAV9 standard material (Rluc) consists of fully assembled AAV9 capsids with a Renilla Luciferase (Rluc) reporter gene controlled by a CMV promotor. Final concentrations (total capsids and viral genome titer) are lot-specific and can be found on the CoA.
Downloads
FAQs
| Serotype | Transfer vector | Packaging plasmid | Helper plasmid |
| AAV2 | AAV2-CMV-Rluc-WPRE-hGHpA | pRep2-cap2 | pHelper |
| AAV5 | AAV5-CMV-Rluc-WPRE-hGHpA | pRep2-cap5 | pHelper |
| AAV6 | AAV6-CMV-Rluc-WPRE-hGHpA | pRep2-cap6 | pHelper |
| AAV8 | AAV8-CMV-Rluc-WPRE-hGHpA | pRep2-cap8 | pHelper |
| AAV9 | AAV9-CMV-Rluc-WPRE-hGHpA | pRep2-cap9 | pHelper |
Related Products
- Consists of fully assembled, empty AAV9 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
- Fully assembled, empty AAV9 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Fully assembled AAV9 capsids filled with an eGFP reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Fully assembled AAV2 capsids filled with an eGFP reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- ELISA for quantitation of AAV9 capsids
- Detection of full and empty AAV9 capsids
- ADK9 antibody is used as capture and detection antibody
- get results in less than 2 hours and increase your daily sample throughput
- easy workflow & process integration
- same accuracy and reproducibility as the standard AAV9 Titration ELISA
- ADK9 antibody is used as capture and detection antibody
- Mouse monoclonal
- Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
- Reacts with denatured VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
- Isotype: IgG1
- Rabbit polyclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
- Strong affinity for denatured capsid proteins
- Weak affinity for native capsids
- Purified, liquid
- Mouse monoclonal
- Suitable for dot blot, ELISA, ICC and neutralization assay
- Reacts with AAV9 intact particles
- Isotype: IgA kappa
- Purified, lyophilized
- Recombinant antibody
- Suitable for dot blot, ELISA and neutralization assay
- Reacts with AAV9 intacts particles
- Isotype: mouse IgG1
- Monoclonal recombinant mouse chimeric antibody
- Suitable for dot blot, IF, ELISA and neutralization
- Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAV4A, AAV4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9 intact particles
- Isotype: mouse IgG2a
- Monoclonal recombinant mouse chimeric antibody
- Suitable for dot blot, IF, ELISA and neutralization
- Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAV4A, AAV4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9 intact particles
- Isotype: mouse IgG2a
- Rapid lateral flow test for the detection of AAV9 capsids
- Semi-quantitative AAV titer determination in cell lysates and purified preparations
- Detection of full and empty AAV9 capsids
- Highly effective density gradient media for the separation of full and empty AAV particles
- Designed for the purification of viruses (such as AAV)
- Useful to isolate different cell types and organelles
- Ready-made, sterile and endotoxin-tested solution of iodixanol