AAVrh74 ELISA Control
- Consists of fully assembled, empty AAVrh74 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
Product description
| Quantity | 1 vial (1.3E+09-2.2E+09 capsids) |
|---|---|
| Application | ELISA |
| Storage | 2-8°C |
| Intended use | Research use only |
| Concentration | 2.6E+09-4.4E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial) |
| Formulation | Lyophilized, empty AAVrh74 capsids. Reconstitute in 500 µl ASSB 1x (provided with PROGEN's AAV ELISAs), incubate 5 min at RT & mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red & ASSB 1x buffer |
| Stability | 4 weeks at 2-8°C after reconstitution in ASSB 1x |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | To generate a standard curve two-fold serial dilutions in ASSB 1x are recommended. For use as a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN's AAV Titration ELISAs) and analysis at least in duplicates is recommended. |
Background
The AAVrh74 ELISA Control consists of fully assembled, empty AAVrh74 capsids. The concentration is lot specific.
The AAVrh74 ELISA Control can be used to generate a standard curve adapting PROGEN's AAVrh10 Titration ELISA (Cat. No. PRAAV10). Two-fold serial dilutions of the AAVrh74 ELISA Control in ASSB 1x buffer (provided with the ELISA kit) result in a typical titration curve. The curve allows the quantitative determination of samples of an unkown particle titer.
The AAVrh74 ELISA control can also be used as a positive control with PROGEN's AAVrh10 ELISA.
The AAVrh74 ELISA Control has been calibrated on an internally established reference standard. The internal reference standard is a preparation of full AAVrh74 capsids that has been characterized by qPCR and ddPCR (DNA quantification) and electron microscopy (ratio of full to empty capsids).
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FAQs
Empty capsids (AAV1, 2, 5, 6, 8, 9, rh10 and rh74) are provided in liquid format in PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl without protein additives. The titer is greater than 5.0E+12 capsids/ml. Therefore, they can be used in several applications.
ELISA Kit Controls (AAV1, 2, 3, 5, 6, 8, 9, rh10 and rh74) are lyophilized. After reconstitution in ASSB 1x the final solution contains stabilizing protein, phenol red and ASSB 1x buffer. The titer is lot-specific around 7.9E+09-1.3E+10 capsids/ml. This product is mainly suitable for the ELISA.
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- Consists of fully assembled, empty AAVrh74 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
- Fully assembled, empty AAVrh74 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Fully assembled AAVrh74 capsids filled with an eGFP reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Purified, lyophilized
- Mouse monoclonal
- Suitable for dot blot, ELISA and neutralization
- Reacts with AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
- Isotype: IgG2a kappa
- Recombinant chimeric (human Fc region)
- Suitable for dot blot, neutralization assay and serology ELISA
- Reacts with AAV3, AAV7, AAV8, AAVrh10 and AAVrh74 intact capsids
- Isotype: IgG1
- Purified, liquid
- Mouse monoclonal
- Suitable for dot blot and ICC/IF
- Reacts with AAV8, AAVrh10, Anc80 and AAVrh74 intact particles
- Weak affinity for AAV9 and AAVDJ intact particles
- Isotype: IgG2a kappa
- Highly effective density gradient media for the separation of full and empty AAV particles
- Designed for the purification of viruses (such as AAV)
- Useful to isolate different cell types and organelles
- Ready-made, sterile and endotoxin-tested solution of iodixanol