Desmin, chicken gizzard, 250 µg

Cat. No: 62005

Available, delivery time 1-7 days

$371.00*

Key Features
  • Purified chicken gizzard desmin
  • Protein standard
Product description
Quantity 250 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Chicken gizzard
Molecular weight 53 kDa
Isoeletric point pI 5.4
Purity > 98% (determined by SDS gelelectrophoresis)
Reconstitution
Reconstitute with 200 µl distilled water (final volume 250 µl).
Final solution: 10 mM sodium phosphate buffer pH 7.5, 6 M urea, 2 mM DTT, 1 mM EDTA, 10 mM methylammonium chloride;
Protein concentration: 1 mg/ml (determined by Bradford method).
Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Background
Protein standard for immunoblotting, immunization and immunoassays.
Reconstitution to filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M and Franke WW (1985). J Cell Biol 101, 1826-1841
- Hatzfeld M et al. (1987). J Mol Biol 197, 237-255
Downloads
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.