Dip’n’Check AAV1 and AAV6 - lateral flow assay
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Concentrations of tolerated buffer additives on the Dip’n’Check AAV1 and AAV6
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Concentrations of tolerated buffer additives on the Dip’n’Check AAV1 and AAV6
- Rapid lateral flow test for the detection of AAV1 and AAV6 capsids
- Semi-quantitative AAV titer determination in cell lysates and purified preparations
- Detection of full and empty AAV1 and AAV6 capsids
Product description
| Quantity | 25 Lateral flow tests |
|---|---|
| Reactivity | AAV1, AAV6 |
| Storage | Box 1: 2-8°C; Box 2: up to 8 weeks at 2-8°C; long term storage at -20°C |
| Intended use | Research use only |
| Product description | Rapid lateral flow test for the detection of AAV1 and AAV6 viral capsids in cell lysates or purified preparations. |
Background
This lateral flow test is a semi-quantitative assay with a detection range of 1.0E+08-1.0E+10 capsids of AAV1 and AAV6 in the assay volume of 156 µl. Within this range, a 2-fold concentration difference is clearly detectable.
Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives
which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.
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FAQs
The lateral flow assay detects 1E+08 to 1E+10 total capsids in the assay volume, which corresponds to a concentration of 1E+09-1E+11 capsids/ml of the starting material.
The Dip’n’Check assay can be used with crude lysate. To find out more about the concentrations of tested tolerated buffer additives take a look in the product manual and go to Section 10 - Matrix Effects or you can also find Table 1 - Concentrations of tolerated buffer additives on the individual product website pages.
No, the Dip’n’Check assay detects all assembled capsids independent of filling grade.
The AAV Dip'n'Check test can be used for a variety of applications:
- To compare AAV titers from different AAV preparations. With this information you can select the most promising sample for further processing.
- To estimate AAV titers in crude and purified AAV preparations. To either control efficiency of your processes or to pre-test AAV concentrations to hit the dynamic range of downstream analytics.
- To test the binding of common AAV antibodies to an AAV capsid variant. Allowing you to confirm which antibody-based methods are suitable for further analysis.
More information about the determination of the Dip’n’Check can be found on our poster: Potential Applications for AAV Lateral Flow Tests
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