Filamin, chicken, 250 µg

Cat. No: 62006

Available, delivery time 1-7 days

$185.00*

Key Features
  • Purified chicken gizzard filamin
  • Protein standard
Product description
Quantity 250 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Chicken gizzard
Molecular weight 250 kDa
Isoeletric point pI 6.4
Purity > 90% (determined by SDS gelelectrophoresis)
Reconstitution Reconstitute with 250 µl Laemmli buffer (final volume 250 µl) or 9 M urea buffer (e.g. IEF or NEPHGE buffer).
Sometimes filamin is not readily soluble after lyophilization. In this case add 250 µl dist. water, after dissolution add 135 mg solid urea (to reach an end concentration of 9 M urea), leave for 1h at room
temperature, separate insoluble rests by a short centrifugation step.
Final solution additionally contains: 20 mM Tris/acetate buffer pH 7.6, 0.1 mM EDTA, 2 mM DTT, 20 mM NaCl;
Protein concentration: 1 mg/ml (according to Bradford)
Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Background
Protein standard for immunoblotting, immunization and immunoassays.
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