Key Features
- Purified chicken gizzard filamin
- Protein standard
Product description
| Quantity | 250 µg |
|---|---|
| Storage | Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles) |
| Intended use | Research use only |
| Source | Chicken gizzard |
| Molecular weight | 250 kDa |
| Isoeletric point | pI 6.4 |
| Purity | > 90% (determined by SDS gelelectrophoresis) |
| Reconstitution | Reconstitute with 250 µl Laemmli buffer (final volume 250 µl) or 9 M urea buffer (e.g. IEF or NEPHGE buffer). Sometimes filamin is not readily soluble after lyophilization. In this case add 250 µl dist. water, after dissolution add 135 mg solid urea (to reach an end concentration of 9 M urea), leave for 1h at room temperature, separate insoluble rests by a short centrifugation step. Final solution additionally contains: 20 mM Tris/acetate buffer pH 7.6, 0.1 mM EDTA, 2 mM DTT, 20 mM NaCl; Protein concentration: 1 mg/ml (according to Bradford) |
| Application | Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization |
Background
Protein standard for immunoblotting, immunization and immunoassays.
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FAQs
Our product chicken Filamin was purified using strong denaturing conditions and HPLC. Therefore we suggest strong denaturing conditions for solubilization of the lyophilized material, e.g. Laemmli buffer (containing SDS) or NEPHGE buffer (containing 9 M urea; cf. our data sheet).
In addition, in solution Filamin has a high tendency for self-aggregation. We therefore strongly suggest addition of urea to the reconstituted product.
Please note, however, that the lyophilized product does not contain urea, the protein was lyophilized in buffer containing: 20 mM Tris/acetate buffer pH 7.6, 0.1 mM EDTA, 2 mM DTT, 20 mM NaCl.
Partial solubilization (in case urea has to be avoided) could be obtained by using the following Detergent-mix buffer: 7% SDS, 3.5% Na-DOC (deoxycholate), 7% Triton X-100 in 150 mM NaCl, 20 mM Tris/acetate buffer (pH 7.6).
We do not recommend a protein concentration of 10 mg/ml. After reconstitution with 250 µl the product has a protein concentration of 1 mg/ml. Higher dilutions should be possible.
Please handle the product in solution always with cooling: According to the literature, Filamin is degrading at room temperature.
In addition, in solution Filamin has a high tendency for self-aggregation. We therefore strongly suggest addition of urea to the reconstituted product.
Please note, however, that the lyophilized product does not contain urea, the protein was lyophilized in buffer containing: 20 mM Tris/acetate buffer pH 7.6, 0.1 mM EDTA, 2 mM DTT, 20 mM NaCl.
Partial solubilization (in case urea has to be avoided) could be obtained by using the following Detergent-mix buffer: 7% SDS, 3.5% Na-DOC (deoxycholate), 7% Triton X-100 in 150 mM NaCl, 20 mM Tris/acetate buffer (pH 7.6).
We do not recommend a protein concentration of 10 mg/ml. After reconstitution with 250 µl the product has a protein concentration of 1 mg/ml. Higher dilutions should be possible.
Please handle the product in solution always with cooling: According to the literature, Filamin is degrading at room temperature.