Filamin, chicken, 250 µg
- Purified chicken gizzard filamin
- Protein standard
|Storage||Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)|
|Intended use||Research use only|
|Molecular weight||250 kDa|
|Isoeletric point||pI 6.4|
|Purity||> 90% (determined by SDS gelelectrophoresis)|
|Reconstitution||Reconstitute with 250 µl Laemmli buffer (final volume 250 µl) or 9 M urea buffer (e.g. IEF or NEPHGE buffer).
Sometimes filamin is not readily soluble after lyophilization. In this case add 250 µl dist. water, after dissolution add 135 mg solid urea (to reach an end concentration of 9 M urea), leave for 1h at room
temperature, separate insoluble rests by a short centrifugation step.
Final solution additionally contains: 20 mM Tris/acetate buffer pH 7.6, 0.1 mM EDTA, 2 mM DTT, 20 mM NaCl;
Protein concentration: 1 mg/ml (according to Bradford)
|Application||Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization|
Protein standard for immunoblotting, immunization and immunoassays.
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