Keratin K18, human recombinant, 100 µg

100 µg

250 µg

Cat. No: 62217

Prices excl. tax

Available, delivery time: 1-7 days

$293.00*

Datasheet

Key Features

Recombinant human keratin K18 (formerly also designated cytokeratin 18)
Protein standard

Product description

Quantity	100 µg
Storage	Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use	Research use only
Source	Human recombinant, produced in E. coli
Molecular weight	45 kDa
Isoeletric point	pI 5.7
Purity	> 95% (determined by SDS gelelectrophoresis)
Reconstitution	Reconstitute with 70 µl distilled water (final volume 100 µl). Final solution: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride; Protein concentration: 1 mg/ml
Application	Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Synonym	Cytokeratin 18

Background

Human recombinant Keratin K18 for use in immunoblotting and ELISA.

Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255

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Datasheet

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FAQs

Reconstitution to filaments is performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.2-0.5 mg/ml, both dissolved in 9.5 M urea buffer: e.g. cytokeratin 18 solution in urea buffer plus cytokeratin 8 solution in urea buffer (both with the same protein concentration!) Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea buffer (for approx. 4 h at RT) and then to low salt condition (overnight at 2-8°C, using e.g. 50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). Use a large excess of dialyzing buffer (e.g. x500). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). For storage of the dialyzed solution we recommend division in suitable aliquots and freezing below -50°C. Avoid repeated freezing / thawing cycles.

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Keratin K18, human recombinant, 100 µg

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How can I get filaments from human recombinant Keratin 18 and Keratin 8?