Keratin K18, human recombinant, 250 µg

Cat. No: 62017

Available, delivery time 1-7 days

$548.00*

Key Features
  • Recombinant human keratin K18 (formerly also designated cytokeratin 18)
  • Protein standard
Product description
Quantity 250 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Human recombinant, produced in E. coli
Molecular weight 45 kDa
Isoeletric point pI 5.7
Purity > 95% (determined by SDS gelelectrophoresis)
Reconstitution Reconstitute with 175 µl distilled water (final volume 250 µl).
Final solution: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride;
Protein concentration: 1 mg/ml
Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Synonym Cytokeratin 18
Background
Human recombinant Keratin K18 for use in immunoblotting and ELISA.
Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
Downloads
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


FAQs
Reconstitution to filaments is performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.2-0.5 mg/ml, both dissolved in 9.5 M urea buffer: e.g. cytokeratin 18 solution in urea buffer plus cytokeratin 8 solution in urea buffer (both with the same protein concentration!) Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea buffer  (for approx. 4 h at RT) and then to low salt condition (overnight at 2-8°C, using e.g. 50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). Use a large excess of dialyzing buffer (e.g. x500). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). For storage of the dialyzed solution we recommend division in suitable aliquots and freezing below -50°C. Avoid repeated freezing / thawing cycles.

Related Products

anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, prediluted, purified
Cat. No: 65031
Quantity: 5 ml
  • Purified, prediluted
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, mouse and rat
  • Isotype: IgG1

Variants from $96.00*
$220.00*
anti-Keratin K7 mouse monoclonal, Ks7.18, liquid, purified, sample
Cat. No: 690025S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, pig and sheep
  • Isotype: IgG1

$96.00*
anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), liquid, purified, sample
Cat. No: 690010S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG2a

$96.00*
anti-Keratin K18 mouse monoclonal, Ks18.04, FITC Conjugate
Cat. No: 61428
Quantity: 250 µl
  • FITC conjugate
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC
  • Reacts with bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish
  • Isotype: IgG1

Variants from $96.00*
$342.00*
anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified
Cat. No: 690029
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human, rabbit, rat
  • Isotype: IgG2b

Variants from $96.00*
$251.00*
anti-Keratin K20 mouse monoclonal, IT-Ks20.8, liquid, purified, sample
Cat. No: 690026S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human and mouse
  • Isotpye: IgG2a

$96.00*
anti-Keratin K17 mouse monoclonal, Ks17.E3, liquid, purified
Cat. No: 690036
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, rat
  • Isotype: IgG2b

Variants from $96.00*
$251.00*
anti-Keratin K8 mouse monoclonal, Ks8.7, liquid, purified
Cat. No: 690038
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with hamster and human
  • Isotype: IgG1

Variants from $96.00*
$251.00*
anti-Keratin K2 mouse monoclonal, Ks2.342.7.4, supernatant
Cat. No: 65191
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with bovine and human
  • Isotype: IgG1

$179.00*
anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant
Cat. No: 65177
Quantity: 1 ml
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG1

$179.00*
anti-Keratin K19 mouse monoclonal, Ks19.10, supernatant
Cat. No: 65135
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human and rat
  • Isotype: IgG1

$191.00*
anti-Keratin K8 mouse monoclonal, Ks8-17.2, supernatant
Cat. No: 65130
  • Mouse monclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, hamster, human and rat
  • Isotype: IgG1

$191.00*
anti-Keratin K18 mouse monoclonal, Ks18.5, supernatant
Cat. No: 65137
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine and human
  • Isotype: IgG1

$191.00*
anti-Keratin K7 mouse monoclonal, OVTL 12-30, supernatant
Cat. No: 18315
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human
  • Isotype: IgG1

$215.00*
anti-Keratin K7 + K17 mouse monoclonal, C46, supernatant
Cat. No: 18313
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with bovine, dog, human and sheep
  • Isotype: IgG1

$215.00*
anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
Cat. No: 610145
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC
  • Reacts with bovine, chicken, human, mouse, pleurodeles, rat, snake and xenopus
  • Isotype: IgG1

$216.00*
anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified
Cat. No: 61054
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human, mouse and pig
  • Isotype: IgG1

Variants from $225.00*
$371.00*
anti-Keratin Pan rabbit polyclonal, purified
Cat. No: 10550
  • Rabbit Polyclonal
  • Suitable for IHC on frozen and  paraffin sections
  • Reacts with human and mouse

$232.00*
anti-Keratin K9 mouse monoclonal, Ks9.70 + Ks9.216, supernatant
Cat. No: 651104
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
  • Isotypes: IgG1 and IgG3

$245.00*
anti-Keratin K13 mouse monoclonal, Ks13.1, lyophilized, purified
Cat. No: 61007
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, rat
  • Isotype: IgG1

Variants from $161.00*
$251.00*
anti-Keratin K18 mouse monoclonal, Ks18.27, lyophilized, purified
Cat. No: 61034
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with bovine and human
  • Isotype: IgG1

$251.00*
anti-Keratin K6 mouse monoclonal, KA12, lyophilized, purified
Cat. No: 61090
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse and rat
  • Isotype: IgG1

Variants from $96.00*
$251.00*
anti-Keratin K18 Multiepitope Cocktail mouse monoclonal, Ks18.04 + 18.27 + 18.5 + 9B1 + 18.174, supernatant
Cat. No: 651134
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mammals
  • Isotype: IgG1

$258.00*
anti-Keratin Type II mouse monoclonal, AE3, purified
Cat. No: 61806
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, chicken, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1

$284.00*