Keratin K8, bovine, 100 µg

Cat. No: 62203

Available, delivery time 1-7 days

$213.00*

Key Features
  • Bovine keratin K8 (formerly also designated cytokeratin 8)
  • Protein standard
Product description
Quantity 100 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Bovine liver
Molecular weight 52.5 kDa
Isoeletric point pI 6.4
Purity > 95% (determined by SDS gelelectrophoresis)
Reconstitution Reconstitute with 70 µl distilled water (final volume 100 µl).
Final solution: 30 mM Tris/HCI pH 8, 9 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride;
Protein concentration: 1 mg/ml
Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Synonym Cytokeratin 8
Background
Protein standard for immunoblotting, immunization, and immunoassays.
Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
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anti-Keratin K8/K18 guinea pig polyclonal, serum
Cat. No: GP11
Quantity: 100 µl
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with bovine, chicken, human, mouse, rat and hamster

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