LymphoPrep™ Tube

Cat. No: 1019818

Available, delivery time 1-7 days

$204.00*

Key Features
  • Sterile tube in which the LymphoPrep™ is contained below a plastic filter disc
  • Useful for the separation of monocytes from whole blood
Product description
Quantity 18 tubes (10 ml)
Intended use Research use only
Product description Lymphoprep ™ is a ready made sterile an endotoxin tested solution for the isolation of human mononuclear cells. The Lymphoprep™ Tube is a sterile plastic tube prefilled with Lymphoprep™. The solution contains sodium diatrizoate and polysaccharide.
The Lymphoprep™ Tube consists of two compartments separated by a plastic insert. The lower compartment is prefilled with Lymphoprep™. The plastic insert allows the blood sample to be poured directly into the tube. No layering of the sample is necessary.
Density 1.077 ± 0.001 g/ml
Composition 9.1% (w/v) Sodium Diatrizoate, 5.7% (w/v) Polysaccharide
Osmolality 290 ± 15 mOsm
Endotoxin < 1.0 EU/ml
Stability & storage Lymphoprep™ Tube is produced under sterile conditions. Lymphoprep is stable for 3 years provided the solution is kept sterile and protected from direct sunlight.
Background

A simple and effective method for the isolation of mononuclear cells from human blood was reported by Dr. Arne Boyum in 1968. For more than 35 years a commercial medium known as LymphoprepTM has been widely used for isolating these cells.

Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than the erythrocytes and the polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an isoosmotic medium with a density of 1.077 g/ml, which allows the erythrocytes and the PMNs to sediment through the medium while retaining the mononuclear cells at the sample/medium interface.

The described method is rapid, simple and reliable and gives excellent results with blood samples from normal individuals and patients.

To obtain the maximum yield it is important that the blood sample is diluted 1:1 with physiological saline before being applied to the gradient.

The contamination of erythrocytes in the mononuclear cell suspension is usually between 3-10% of the total cell number.

Some immature PMNs may band with the lymphocytes during intense immunosuppressive therapy. When heparinised blood is used, it is essential to remove most of the platelets, in order to avoid inhibition in the cytotoxicity test.

LymphoprepTM can be used for the preparation of pure lymphocyte suspensions for tissue typing, antilymphocyte sera and immunological research. Thorsby and Brattelie used this technique with only slight modifications in the preparation of pure lymphocyte suspensions for cytotoxicity tests and lymphocyte cultures.

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