Positive western blot control: anti-Keratin K18 antibody
- Whole cell lysate from MCF7 human breast adenocarcinoma cell line
- Validated and tested western blot control for: anti-Keratin K18 mouse monoclonal antibody, clone Ks18.04, Cat. No. 61028 and Cat. No. 65028
|Cell line/ tissue||MCF7|
|Storage||Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)|
|Intended use||Research use only|
|Species of origin||Human|
|Formulation||Lyophilized, reconstitute in 50 µl 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)|
|Tested positive control for||anti-Keratin K18 mouse monoclonal antibody, clone Ks18.04, Cat. No. 61028 and Cat. No. 65028|
|Tested applications||Tested dilutions|
|Western Blot (WB)||5-10 µg total protein per lane|
Whole cell lysate from MCF7 human breast adenocarcinoma cell line.
Tested and validated western blot control for:
anti-Keratin K18 mouse monoclonal antibody, clone Ks18.04, Cat. No. 61028
MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.
Q & A's
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Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Purified, lyophilized
- Mouse monoclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish
- Isotype: IgG1