Positive western blot control: anti-Vimentin antibody
- Whole cell lysate from SV80 human lung fibroblast (SV40-transformed) cell line
- Validated and tested western blot control for: anti-Vimentin mouse monoclonal antibody, VIM 3B4, Cat. No. 61013
|Cell line/ tissue||SV80|
|Storage||Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)|
|Intended use||Research use only|
|Species of origin||Human|
|Formulation||Lyophilized, reconstitute in 50 µl 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)|
|Tested positive control for||anti-Vimentin mouse monoclonal antibody, VIM 3B4, Cat. No. 61013|
|Tested applications||Tested dilutions|
|Western Blot (WB)||10 µg total protein per lane|
Whole cell lysate from SV80 human lung fibroblast (SV40-transformed) as positive western blot control.
Tested and validated western blot control for:
anti-Vimentin mouse monoclonal antibody, VIM 3B4, Cat. No. 61013.
SV80 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.
Q & A's
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Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Purified, lyophilized
- Mouse monoclonal
- Suitable for ELISA, ICC/IF, IHC and WB
- Reacts with amphibia, bovine, chicken, human, mouse and monkey
- Isotype: IgG2a