Non-viral delivery systems for gene therapy
Delivering genetic material safely and efficiently into cells has long been a major challenge in the gene therapy community. Traditional methods, like viral vectors, often carry risks such as immune reactions and toxicity, while overcoming complex manufacturing and scalability challenges still remains difficult.
Lipid Nanoparticles (LNPs) are widely known as drug delivery vehicles composed of lipids and attracted attention for RNA vaccines against COVID-19. However, they also have potential as a non-viral, safe, and robust delivery system for gene therapy. LNPs protect fragile nucleic acids, ensure efficient delivery, and overcome biological barriers, making gene therapies more accessible, scalable, and safer for widespread medical use.
In Vivo Assays
- Evaluation of biodistribution
- Therapeutic evaluation
- Cell tracking
In Vitro Assays
- Labeling of live cells
- Tracking cell internalization
- FRET studies
- Intracellular delivery of therapeutic compounds
- Therapeutic evaluation
- Cytotoxicity studies
Your LNP Delivery Kit Benefits
Stable:
In a variety of biological fluids, sera and cell culture media, while showing narrow size distribution.
High loading capacity:
Ensuring the desired therapeutic effect.
Efficient intracellular delivery:
Overcoming biological barriers.
Versatile:
Able to carry a wide range of molecules.
Easy-to-use:
Formulations are prepared by simply pipetting. No equipment needed.
Suitable for both in vitro & in vivo applications:
Biocompatible with in vitro experiments with different cell types and in vivo models such as mice, rats and zebrafish.
Fluorescent option available:
Real-time tracking of loaded nanoparticles in vitro is facilitated by a built-in fluorescent tag conjugated to the sphingolipids (Ex/Em = 495/503 nm), enabling both cell internalization monitoring and FRET studies.
Nanoparticle Reagents for Small Molecule Delivery
Nanoparticle reagent kits enhancing the solubility of hydrophobic and amphiphilic compounds.
- Robust protection against degradation with high loading capacity
- Significant enhancement of pharmacokinetics & overall drug efficacy
- Efficient intracellular delivery, minimal cytotoxicity
Nanoparticle Reagents for Peptide Delivery
The kits ensure efficient delivery of anionic peptides, essential for the development of targeted & advanced therapies.
- Optimized for negatively charged peptides
- Ideal for proteomics, peptide drug development & targeted therapy research
- Preserving peptide integrity & activity
COMING SOON
Nanoparticle Reagents for Protein Delivery
The kits are specifically designed for precision biomedicine and therapeutic evaluation.
- Non-toxic, without cationic lipids
- Keeps native protein structure and function;
- For protein engineering, targeted therapies & regenerative medicine
Nanoparticle Reagents for mRNA Delivery
A breakthrough in gene therapy and molecular biology enabling efficient mRNA transfection in cells, including primary cultures and organoids.
- Highly biocompatible and efficient: strong expression without cationic lipids
- RNA therapeutics, precision medicine & in vitro/in vivo gene expression studies
- Fluorescent options and customized reagents available for delivery of siRNA, miRNA, circRNA & ASOs
Customized formulations: your path to targeted delivery
We offer comprehensive customization solutions to optimize your cargo delivery.
Upon request, our LNPs can feature:
- Green or cyan fluorophores
- Surface-decoration with tissue-specific ligands
- Different concentration of charged lipids, enabling targeted delivery.
Aditionally, our nanoparticles can be tailored to uptake different molecules within the same nanoparticle, including:
- Combinations of nucleic acids (e.g. siRNA and an aptamer)
- Nucleic acids and small drugs (e.g. aptamer and small drug)
- 2 different small drugs
- Nucleic acids and proteins (e.g. sgRNA and Cas9)
- Nucleic acids and a Polysaccharide (e.g. plasmid and hyaluronic acid)
- Up to 4 different nucleic acids (e.g sgRNA, mRNA1, mRNA2, and DNA)
