Key Features
- Mouse monoclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
- Isotype: IgG2a
Product description
Quantity | 200 µl |
---|---|
Antibody Type | Monoclonal |
Host | Mouse |
Isotype | IgG2a |
Conjugate | Unconjugated |
Application | ELISA, ICC/IF, IP, WB |
Purification | Affinity chromatography |
Reactivity | AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVDJ |
Storage | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Clone | A1 |
Immunogen | AAV2 capsids |
Concentration | 50 µg/ml |
Formulation | PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
ELISA | Assay dependent |
Immunoprecipitation (IP) | Assay dependent |
Western Blot (WB) | 1:500 (0.1 µg/ml) |
Background
A1 reacts with VP1 of adeno-associated virus 1-9 and DJ (AAV1-9, DJ). In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. Epitope mapping experiments (Wobus et al. 2000) identified aa123 to aa131 of VP1 capsid protein as the specific binding region.
Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
References/Publications (5)
Publication
Species
Application
Species
AAV1, 2. 5. 8
Application
dot blot
Species
AAV1,AAV2,AAV3,AAV4,AAV5,AAV6,AAV7,AAV8,AAV9,AAVrh10,AAV11,AAV12
Application
WB
Species
AAV2
Application
WB
Species
AAV2
Application
epitope mapping
Species
AAV2
Application
WB,ICC-IF
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