anti-AAV VP1, mouse monoclonal, A1

Liquid

Liquid

Cat. No: 690056

Available, delivery time 1-7 days

$468.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

Please note that the buffer was changed from PBS to PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA to improve the stability of the antibody. This product replaces the former product with Cat. No. 61056.

Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application ELISA, ICC/IF, IP, WB
Purification Affinity chromatography
Reactivity AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVDJ
Storage Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone A1
Immunogen AAV2 capsids
Concentration

50 µg/ml 

Formulation PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
ELISA Assay dependent
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:500 (0.1 µg/ml)
Background

A1 reacts with VP1 of adeno-associated virus 1-9 and DJ (AAV1-9, DJ). In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. Epitope mapping experiments (Wobus et al. 2000) identified aa123 to aa131 of VP1 capsid protein as the specific binding region.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

References/Publications (4)
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