Conformational epitope
Neutralizing
Recombinant

anti-AAV2 (intact particle) mouse recombinant, A20R, Biotin Conjugate

Lyophilized

Lyophilized

Biotin

Cat. No: 615298

Available, delivery time 1-7 days

$287.00*

Key Features
  • Biotin conjugate
  • Recombinant antibody
  • Suitable for dot blot, ELISA, neutralization assay
  • Reacts with AAV2, AAV3, AAV2 7m8 and Anc80 intact particles
  • Isotype: mouse IgG1
Product description
Quantity 750 µl
Antibody Type Recombinant
Host Mouse
Isotype IgG1
Conjugate Biotin
Application Dot blot, ELISA
Purification Affinity chromatography
Reactivity AAV2, AAV2 7m8, AAV3, Anc80
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution 2-8°C
Intended use Research use only
Clone A20R
Immunogen AAV2 capsids
Formulation Lyophilized; reconstitute in 750 µl sterile PBS
Synonym Adeno-associated virus 2; AAV-2
Applications
Tested applications Tested dilutions
Dot Blot 1:100 (0.5 µg/ml; non-denaturing conditions)
ELISA 1:20
Background

For characterization of different stages of infection and very useful for the analysis of the AAV2 assembly process. A20R specifically reacts with intact AAV2, AAV3 and Anc80 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.
The A20R antibody recognizes the same epitope as the A20 antibody (Cat. No. 61055).

Limited Use Label License: Research Use Only
Product is exclusively owned by PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

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FAQs
A20 is a mouse IgG3 monoclonal antibody, while A20R is recombinant mouse IgG1 antibody. Both antibodies recongize the same epitope, only the antibody backbone is different.
There is no difference in performance.

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

AAV3 ELISA Control
Cat. No: PRAAV3-C
  • Consists of fully assembled, empty AAV3 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
AAV2 ELISA Control
Cat. No: PRAAV2-C
  • Consists of fully assembled, empty AAV2 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
OptiPrep™ (Iodixanol)
Cat. No: 1114542
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

$195.00*
anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified
Cat. No: 61055
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA, ICC and neutralization assay
  • Reacts with AAV2, AAV2 7m8 and AAV3 intact particles
  • Isotype: IgG3

Variants from $131.00*
$461.00*
AAV3 Titration ELISA 2.0R
Cat. No: PRAAV3R
  • ELISA for quantitation of AAV3 capsids
  • Detection of full and empty AAV3 capsids
  • A20R antibody is used as capture and detection antibody

$677.00*
AAV2 Titration ELISA 2.0R
Cat. No: PRAAV2R
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20R antibody is used as capture and detection antibody

$677.00*
AAV2 Xpress ELISA
Cat. No: PRAAV2XP
Fast, easy and reliable AAV2 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV2 Titration ELISAs
  • A20R antibody is used as capture and detection antibody

$825.00*