Conformational epitope
Frequently used
Neutralizing

anti-AAV4 (intact particle) mouse monoclonal, ADK4, lyophilized, purified

Lyophilized

Biotin

Cat. No: 610147

Available, delivery time 1-7 days

$438.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and ICC
  • Reacts with AAV4 intact particles
  • Isotype: IgG2a kappa
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application Affinity chromatography, Dot blot, ELISA, ICC/IF, IP
Purification Affinity chromatography
Reactivity AAV4
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone ADK4
Immunogen AAV4 capsids
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Synonym Adeno-associated virus 4; AAV-4
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Affinity Chromatography Assay dependent
Dot Blot 1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA Assay dependent
Immunoprecipitation (IP) 1:5
Background

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process.
ADK4 specifically reacts with intact adeno-associated virus 4 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.
The antibody cannot be used for immunoblotting.

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OptiPrep™ (Iodixanol)
Cat. No: 1114542
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

$195.00*