Conformational epitope
Frequently used

anti-AAV5 (intact particle) mouse monoclonal, ADK5b, lyophilized, purified, sample



Cat. No: 610149S

Available, delivery time 1-7 days


Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, ICC and neutralization assays
  • Reacts with AAV5 intact capsids
  • Isotype: IgG2b kappa
Product description
Quantity 10 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b kappa
Conjugate Unconjugated
Application Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification Affinity chromatography
Reactivity AAV5
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone ADK5b
Immunogen AAV5 capsids
Concentration 50 µg/ml after reconstitution with 200 µl PBS
Formulation Lyophilized; reconstitute in 200 µl sterile PBS
Synonym Adeno-associated Virus 5, AAV-5
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Dot Blot 1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA Assay dependent
Immunoprecipitation (IP) 1:5
Neutralization Assay Assay dependent

For characterization of different stages of infection and very useful for the analysis of the AAV5 assembly process. ADK5b specifically reacts with intact adeno-associated virus 5 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
The antibody is usefull for neutralizing experiments.

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

References/Publications (3)
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Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

AAV5 ELISA Control
Cat. No: PRAAV5-C
  • Consists of fully assembled, empty AAV5 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

OptiPrep™ (Iodixanol)
Cat. No: 1114542
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

anti-AAV5 (intact particle) mouse monoclonal, ADK5a, lyophilized, purified
Cat. No: 610148
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization assay
  • Reacts with AAV5 intact capsids
  • Isotype: IgG2a kappa

Variants from $131.00*
anti-AAV5 rabbit polyclonal, serum
Cat. No: 610137
  • Rabbit polyclonal
  • Suitable for Affinity Chromatography, ICC/IF and WB
  • Reacts with AAV5

AAV5 Titration ELISA
Cat. No: PRAAV5
  • ELISA for quantitation of AAV5 capsids
  • Detection of full and empty AAV5 capsids
  • ADK5a antibody is used as capture and detection antibody