Dot blot analysis of native AAV1-AAV9, AAVrh10, AAVDJ capsids (1E+09-1E+10 capsids) and denatured AAV8 capsids (1E+09-1E+10 capsids, denatured at 95°C for 10 min in sample buffer). The nitrocellulose membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9 (Cat. No. 690161) was diluted in blocking buffer (antibody concentration 1 ug/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG HRP was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Plus Western Blotting Substrate.
Dot blot analysis of native AAV8, Anc80 and AAVrh74 capsids (1E+09 capsids). The nitrocellulose membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9 (Cat. No. 690161) was diluted in blocking buffer (antibody concentration 500 ng/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG goat HRP was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Plus Western Blotting Substrate.
Dot blot analysis of native AAV1-AAV9, AAVrh10, AAVDJ capsids (1E+09-1E+10 capsids) and denatured AAV8 capsids (1E+09-1E+10 capsids, denatured at 95°C for 10 min in sample buffer). The nitrocellulose membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9 (Cat. No. 690161) was diluted in blocking buffer (antibody concentration 1 ug/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG HRP was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Plus Western Blotting Substrate.
Dot blot analysis of native AAV8, Anc80 and AAVrh74 capsids (1E+09 capsids). The nitrocellulose membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9 (Cat. No. 690161) was diluted in blocking buffer (antibody concentration 500 ng/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG goat HRP was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Plus Western Blotting Substrate.
Key Features
- Purified, liquid, sample
- Mouse monoclonal
- Suitable for dot blot and ICC/IF
- Reacts with AAV8, AAVrh10, Anc80 and AAVrh74 intact particles
- Weak affinity for AAV9 and AAVDJ intact particles
- Isotype: IgG2a kappa
Product description
| Quantity | 200 µl (50 µg/ml) |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG2a kappa |
| Conjugate | Unconjugated |
| Application | Dot blot, ICC/IF, IP |
| Purification | Affinity chromatography |
| Reactivity | AAV8, AAV9, AAVDJ, AAVrh10, AAVrh74, Anc80 |
| No reactivity | AAV1, AAV2, AAV3, AAV4, AAV5, AAV6 |
| Storage | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | ADK8/9 |
| Immunogen | AAV8 capsids |
| Concentration | 50 µg/ml |
| Formulation | 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4 |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Dot Blot | 1:50-1:250 (0.2-1 µg/ml; non-denaturing conditions) |
| Immunoprecipitation (IP) | Assay dependent |
Background
For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK8/9 specifically reacts with AAV8, AAVrh10, Anc80 and AAVrh74 and with weak affinity with AAV9 and AAVDJ, empty and full capsids. Recognizes a conformational epitope of assembled capsids.
The antibody cannot be used for immunoblotting using denaturing conditions.
References/Publications (4)
Publication
Species
Application
Species
AAV9
Application
IP
Species
AAV8
Application
cryoEM
Species
AAV8
Application
ICC-IF
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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