Conformational epitope
Highly published
Neutralizing

anti-AAV8 (intact particle) mouse monoclonal, ADK8, lyophilized, purified, sample

Lyophilized

Lyophilized

Supernatant

Biotin

Cat. No: 610160S

Available, delivery time 1-7 days

$133.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization
  • Reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
  • Isotype: IgG2a kappa
Product description
Quantity 10 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification Affinity chromatography
Reactivity AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74, Anc80
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone ADK8
Immunogen AAV8 capsids
Concentration 50 µg/ml after reconstitution with 200 µl PBS
Formulation Lyophilized; reconstitute in 200 µl sterile PBS
Synonym Adeno-associated virus 8, AAV-8
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Dot Blot 1:100-1:500 (0.1-0.5 µg/ml; non-denaturing conditions)
ELISA Assay dependent
Immunoprecipitation (IP) Assay dependent
Neutralization Assay Assay dependent
Background

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK8 specifically reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Predicted binding site: residues 586-LQQQNT-591 (Gurda et al. 2012). The antibody cannot be used for immunoblotting using denaturing conditions.
The antibody is also useful for neutralizing experiments.
Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol. 86, 7739-7751 (2012).

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

References/Publications (6)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes


EM structures of assembled AAV1, 2, 4, 5, 6, and 8 capsids with Fab-fragments are available in the following publications:


PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
AAV8 ELISA Control
Cat. No: PRAAV8-C
  • Consists of fully assembled, empty AAV8 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$137.00*
AAV8 empty capsids
Cat. No: 66V080
  • Fully assembled, empty AAV8 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

$300.00*
AAV8 Titration ELISA
Cat. No: PRAAV8
  • ELISA for quantitation of AAV8 capsids
  • Detection of full and empty AA8 capsids
  • ADK8 antibody is used as capture and detection antibody

$687.00*
AAV8 Xpress ELISA
Cat. No: PRAAV8XP
Fast, easy and reliable AAV8 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV8 Titration ELISA
  • ADK8 antibody is used as capture and detection antibody

$837.00*
AAVrh10 Titration ELISA
Cat. No: PRAAV10
  • ELISA for quantitation of AAVrh10 capsids
  • Detection of full and empty AAVrh10 capsids
  • ADK8 antibody is used as capture and detection antibody

$687.00*
anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified
Cat. No: 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

$468.00*
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
Cat. No: 690058
Quantity: 1 ml
  • Mouse monoclonal
  • Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
  • Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1

Variants from $130.00*
$457.00*
anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No: 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11 and AAV12

$468.00*
anti-AAV8 (intact particle) mouse monoclonal, ADK8, lyophilized, purified
Cat. No: 610160
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization
  • Reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
  • Isotype: IgG2a kappa

Variants from $133.00*
$445.00*
anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9, liquid, purified
Cat. No: 690161
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for dot blot and ICC/IF
  • Reacts with AAV8, AAV9, AAVrh10, AAVDJ, Anc80 and AAVrh74 intact particles
  • Isotype: IgG2a kappa

Variants from $133.00*
$445.00*
Dip’n’Check AAV8
Cat. No: PR5208
  • Rapid lateral flow test for the detection of AAV8 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV8 capsids

$204.00*
OptiPrep™ (Iodixanol)
Cat. No: 1893 (previous 1114542)
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

Please note: The Cat. No. of OptiPrepTM changes from 1114542 to 1893 but the product itself has not been changed in any way.
Therefore it is possible to receive the ordered products with Cat. No. 1114542 or 1893.

$198.00*