Conformational epitope
Highly published
Neutralizing

anti-AAV9 (intact particle) mouse monoclonal, ADK9, liquid, purified, sample

Liquid

Supernatant

Liquid

Biotin

Cat. No: 690162S

Available, delivery time 1-7 days

$133.00*

Key Features
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for dot blot, ELISA, ICC and neutralization assay
  • Reacts with AAV9 intact particles
  • Isotype: IgA kappa
Product description
Quantity 200 µl (50 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgA kappa
Conjugate Unconjugated
Application Dot blot, ELISA, ICC/IF, Neutralization assay
Purification Affinity chromatography
Reactivity AAV9
Storage Up to 1 month: 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone ADK9
Immunogen AAV9 capsids
Concentration 50 µg/ml
Formulation Liquid; PBS with 0.09% sodium azide and 0.5% BSA
Synonym Adeno-associated virus 9; AAV-9
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Dot Blot 1:100-1:1,000 (0.05-0.5 µg/ml; non-denaturing conditions)
ELISA Assay dependent
Neutralization Assay Assay dependent
Background
For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK9 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. The antibody cannot be used for immunoblotting.
The antibody is also useful for neutralizing experiments.

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

References/Publications (4)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

ADK9 is a mouse monoclonal IgA antibody, while ADK9-1R is a recombinant mouse IgG1 antibody. Both antibodies recongize the same epitope, only the antibody backbone is different.
There is no difference in performance.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9, supernatant
Cat. No: 651161
Quantity: 5 ml
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for dot blot and ICC/IF
  • Reacts with AAV8, AAV9, AAVrh10, AAVDJ, Anc80 and AAVrh74 intact particles
  • Isotype: IgG2a kappa

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anti-AAV9 (intact particle) mouse recombinant, ADK9-1R, lyophilized, purified
Cat. No: 610178
Quantity: 50 µg
  • Purified, lyophilized
  • Recombinant antibody
  • Suitable for dot blot, ELISA and neutralization assay
  • Reacts with AAV9 intacts particles
  • Isotype: mouse IgG1

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AAV9 Titration ELISA
Cat. No: PRAAV9
  • ELISA for quantitation of AAV9 capsids
  • Detection of full and empty AAV9 capsids
  • ADK9 antibody is used as capture and detection antibody

$687.00*