Epitope mapped
Highly published
Diagnostic marker

anti-Actin mouse monoclonal, 2G2, supernatant

Cat. No: 651132

Available, delivery time 1-7 days

$293.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, chicken, human, plant and rabbit
  • Isotype: IgM
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgM
Conjugate Unconjugated
Purification Hybridoma cell culture supernatant
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 2G2
Immunogen Profilin actin complex from calf thymus
Formulation Contains 0.09% sodium azide
UniprotID Q3B7N2 (Bovine), P05094 (Chicken), P12814 (Human), A0A5F9CV24 (Rabbit)
Synonym Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent (IF only after methanol fixation)
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (protease treatment recommended)
Western Blot (WB) Assay dependent
Background

Specific for skeletal and non-muscle actin isoforms (43 kDa). Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.

When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic actin was only positive after fixation with methanol (Gonsior et al., 1999).

Reactivity on cultured cell lines: PtK2, SR-NRK, NRK-49F, L6 cells, rat fibroblasts, NIH-3T3, mouse myoblast and myotube cells.

Gonsior SM, Platz S, Buchmeier S, Scheer U, Jockusch BM, Hinssen H: Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J Cell Sci 112, 797-809 (1999)

References/Publications (9)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Actin, rabbit, 250 µg
Cat. No: 62001
Quantity: 250 µg
  • Purified rabbit actin
  • Protein standard

Variants from $123.00*
$185.00*
anti-Actin mouse monoclonal, 647, supernatant
Cat. No: 10601
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with chicken, human, mouse and rat
  • Isotype: IgM

$306.00*