Diagnostic marker

anti-Alpha fetoprotein (AFP) mouse monoclonal, C3, purified

Cat. No: 691543

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF and IHC
  • Reacts with dog, human, monkey and pig
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Dog, Human, Monkey, Pig
No reactivity Bovine, Mouse, Rat
Storage 2-8°C
Intended use Research use only
Clone C3
Immunogen Alpha fetoprotein isolated from serum of hepatoma patients
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID Q8MJU5 (Dog, Canis familiaris), P02771 (Human), Q8MJ76 (Pig)
Synonym Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml

C3 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nunstad to a group of antibodies with low affinity to human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa and expressed in fetal liver, but normally absent in healthy adult tissues. It is expressed in all yolk sac tumors, in some other germ cell tumors and in hepatocellular carcinomas.

Positive control: Hep-G2 cells, fetal liver or hepatocellular carcinoma.

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