anti-Bromodeoxyuridine (BrdU) mouse monoclonal, ascites

Cat. No: 11200

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for FACS and IHC
  • Reacts with all species
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application FACS, IHC
Purification Ascites
Reactivity All species
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone IIB5
Immunogen BrdU conjugated to BSA
Formulation Contains 0.09% sodium azide
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10 (proteolytic treatment with pepsin)
Immunohistochemistry (IHC) - paraffin 1:10 (proteolytic treatment with pepsin)
Flow Cytometry (FACS) Assay dependent

IIB5 reacts with BrdU in denatured (single-stranded DNA). The antibody is crossreactive with iododeoxyuridine. It can be used for:
1. Radioimmunocytochemical detection of circulating levels of BrdU
2. Detection of S-phase cells in tissue sections by immunoperoxidase or immunofluorescence method
3. Detection of S-phase cells in cell suspension
4. Determination of the percentage of profilerating cells by flow cytometry
5. Quantitative determination of the number of various phases of the cell cycle by dual parameter flow-cytometrical analysis

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anti-Bromodeoxyuridine mouse monoclonal, Bu5.1, lyophilized, purified
Cat. No: 61015
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with tissue and cell culture after incorporation of BrdU
  • Isotype: IgG2a