anti-Bromodeoxyuridine (BrdU) mouse monoclonal, ascites
anti-Bromodeoxyuridine (BrdU) mouse monoclonal, ascites
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Memi, F., Killen, A. C., et al. Cadherin 8 regulates proliferation of cortical interneuron progenitors. Brain Struct Funct. 2019-01-01.
Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Memi, F., Killen, A. C., et al. Cadherin 8 regulates proliferation of cortical interneuron progenitors. Brain Struct Funct. 2019-01-01.
Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Memi, F., Killen, A. C., et al. Cadherin 8 regulates proliferation of cortical interneuron progenitors. Brain Struct Funct. 2019-01-01.
Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
anti-Bromodeoxyuridine (BrdU) mouse monoclonal, ascites
View more
Memi, F., Killen, A. C., et al. Cadherin 8 regulates proliferation of cortical interneuron progenitors. Brain Struct Funct. 2019-01-01.
Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Memi, F., Killen, A. C., et al. Cadherin 8 regulates proliferation of cortical interneuron progenitors. Brain Struct Funct. 2019-01-01.
Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Memi, F., Killen, A. C., et al. Cadherin 8 regulates proliferation of cortical interneuron progenitors. Brain Struct Funct. 2019-01-01.
Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Key Features
- Mouse monoclonal
- Suitable for IHC
- Reacts with all species
- Isotype: IgG1
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | IHC |
| Purification | Ascites |
| Reactivity | All species |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | IIB5 |
| Immunogen | BrdU conjugated to BSA |
| Formulation | Contains 0.09% sodium azide |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:10 (proteolytic treatment with pepsin) |
| Immunohistochemistry (IHC) - paraffin | 1:10 (proteolytic treatment with pepsin) |
Background
IIB5 reacts with BrdU in denatured (single-stranded DNA). The antibody is crossreactive with iododeoxyuridine. It can be used for:
1. Radioimmunocytochemical detection of circulating levels of BrdU
2. Detection of S-phase cells in tissue sections by immunoperoxidase or immunofluorescence method
3. Detection of S-phase cells in cell suspension
4. Determination of the percentage of profilerating cells by flow cytometry
5. Quantitative determination of the number of various phases of the cell cycle by dual parameter flow-cytometrical analysis
References/Publications (5)
Publication
Species
Application
Species
BrdU
Application
IHC (frozen)
Species
BrdU
Application
IHC (frozen)
Species
BrdU
Application
ICC
Species
BrdU
Application
IHC (paraffin)
Species
BrdU
Application
IHC (paraffin)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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anti-Bromodeoxyuridine mouse monoclonal, Bu5.1, lyophilized, purified
$251.00*
Cat. No: 61015
- Mouse monoclonal
- Suitable for ICC/IF and IHC
- Reacts with tissue and cell culture after incorporation of BrdU
- Isotype: IgG2a