Diagnostic marker

anti-CD45 mouse monoclonal, 135-4C5, purified

Cat. No: 691585

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG2b
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone 135-4C5
Immunogen Stimulated human leucocytes
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:25-1:50 (2-4 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:25-1:50 (2-4 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 1-2 µg/million cells
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

CD45 glycoproteins have various molecular weights on various cell types: B-cells 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Isoforms are produced by alternative splicing. Various isoforms are expressed differently on different lymphocytes. All hematopoietic cells express CD45 proteins except erythrocytes. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). 135-4C5 was clustered in Vienna at the IV international Workshop on human leucocyte differentiation antigens.

Positive control: tonsil, Ramos, U-698, or GA-10 cells.

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