Diagnostic marker

anti-CD45R0 mouse monoclonal, EBS-CD-027, purified

Cat. No: 691586

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone EBS-CD-027
Immunogen Lentil lectin purified glycoproteins from leucocytes from a B-cell CCL patient
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P08575 (Human)
Synonym Receptor-type tyrosine-protein phosphatase C, EC 3.1.3.48, Leukocyte common antigen, L-CA, T200, CD antigen CD45, PTPRC, CD45
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). EBS-CD-027 reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B-cells or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK-cells or B-cell lymphomas.

Positive control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in tonsil or reactive lymph node.

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