Disease related

anti-CD45RA mouse monoclonal, 158-4D3, purified

Cat. No: 691587

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone 158-4D3
Immunogen Stimulated human leucocytes
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P08575 (Human)
Synonym Receptor-type tyrosine-protein phosphatase C, EC 3.1.3.48, Leukocyte common antigen, L-CA, T200, CD antigen CD45, PTPRC, CD45
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

CD45 glycoprotein have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cell multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45 RB (exon 5, CD45RC (exon 6) and CD45R or CD45RO (exons 4-6 spliced out). 158-4D3 was clustered in Boston at the Vth International CD workshop as anti CD45RA, useful to differentiate "naive" T-cells from activated (CD45R0+ve) T-cells, or to differentiate T-cell lymphomas (CD45R0+ve) from B-cell lymphomas (CD45RA+ve).

Positive control: Daudi, Raji, IM9 and human lymphocytes. Human lymph node and tonsil.

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