anti-CD53 mouse monoclonal, 63-5A3, purified

Cat. No: 691592

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human
  • Isotype: IgG2b kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone 63-5A3
Immunogen Human Sezary cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P19397 (Human)
Synonym Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25, CD antigen CD53, CD53, MOX44, TSPAN25
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

CD53 is a 33-55 kDa protein expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on B- an T-cells from every stage of differentiation, but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. 161-2 was typed in Kobe, Japan at the VIth International Workshop on human leucocyte differentiation antigens.

Positive control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leucocytes, human tonsil and lymph node.

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